Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression of Pseudomonas aeruginosa In Vitro.
Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression of Pseudomonas aeruginosa In Vitro.
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负压对铜绿假单胞菌体外增殖、毒力因子分泌、生物膜形成和毒力调控基因表达的影响
DOI：
10.1155/2016/7986234
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发表时间：
2016
影响因子：
--
通讯作者：
Tang PF
中科院分区：
文献类型：
--
作者：
Wang GQ;Li TT;Li ZR;Zhang LC;Zhang LH;Han L;Tang PF
客观的。研究NPWT诱导的负压条件对铜绿假单胞菌的影响。方法。实验组铜绿假单胞菌在-125毫米汞柱负压的Luria-Bertani培养基中培养24小时，对照组在大气压下培养。 24小时后测量铜绿假单胞菌菌落的直径。采用ELISA试剂盒、苔黑酚法和弹性蛋白-刚果红测定法对毒力因子进行定量。通过使用伴刀豆球蛋白 A (Con A) 的 Alexa Fluor® 647 缀合物染色来观察生物膜的形成。通过定量RT-PCR测定毒力调节基因。结果。与对照组相比，负压抑制了铜绿假单胞菌的生长。负压下实验组的菌落大小显着小于对照组（p < 0.01）。此外，负压组毒力因子总量减少，包括外毒素A、鼠李糖脂和弹性蛋白酶。 RT-PCR结果显示毒力调节基因的表达水平显着受到抑制。结论。负压能显着抑制铜绿假单胞菌的生长。它导致毒力因子分泌减少、生物膜形成减少以及毒力调节基因表达水平降低。
Objective. To investigate the effect of negative pressure conditions induced by NPWT on P. aeruginosa. Methods. P. aeruginosa was cultured in a Luria–Bertani medium at negative pressure of −125 mmHg for 24 h in the experimental group and at atmospheric pressure in the control group. The diameters of the colonies of P. aeruginosa were measured after 24 h. ELISA kit, orcinol method, and elastin-Congo red assay were used to quantify the virulence factors. Biofilm formation was observed by staining with Alexa Fluor® 647 conjugate of concanavalin A (Con A). Virulence-regulated genes were determined by quantitative RT-PCR. Results. As compared with the control group, growth of P. aeruginosa was inhibited by negative pressure. The colony size under negative pressure was significantly smaller in the experimental group than that in the controls (p < 0.01). Besides, reductions in the total amount of virulence factors were observed in the negative pressure group, including exotoxin A, rhamnolipid, and elastase. RT-PCR results revealed a significant inhibition in the expression level of virulence-regulated genes. Conclusion. Negative pressure could significantly inhibit the growth of P. aeruginosa. It led to a decrease in the virulence factor secretion, biofilm formation, and a reduction in the expression level of virulence-regulated genes.
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影响因子：
5.5
作者：
Hsu, Chih-Chin;Tsai, Wen-Chung;Wang, Jong-Shyan
通讯作者：
Wang, Jong-Shyan
影响因子：
3.7
作者：
Liu Y;Li Y;Liu K;Shen J
通讯作者：
Shen J
DOI：
10.1097/00004630-199209000-00003
发表时间：
1992-01-01
期刊：
Journal of Burn Care and Rehabilitation
影响因子：
--
作者：
Heggers, John P.;Haydon, Scott;Robson, Martin C.
通讯作者：
Robson, Martin C.
影响因子：
3.8
作者：
Schmidtchen, A;Holst, E;Björck, L
通讯作者：
Björck, L
影响因子：
3.2
作者：
Pearson, JP;Pesci, EC;Iglewski, BH
通讯作者：
Iglewski, BH