Nerve Growth Factor, Brain-Derived Neurotrophic Factor and Osteocalcin Gene Relationship in Energy Regulation, Bone Homeostasis and Reproductive Organs Analyzed by mRNA Quantitative Evaluation and Linear Correlation Analysis.
Nerve Growth Factor, Brain-Derived Neurotrophic Factor and Osteocalcin Gene Relationship in Energy Regulation, Bone Homeostasis and Reproductive Organs Analyzed by mRNA Quantitative Evaluation and Linear Correlation Analysis.
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DOI：
10.3389/fphys.2016.00456
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发表时间：
2016
影响因子：
4
通讯作者：
Tricarico D
中科院分区：
文献类型：
--
作者：
Camerino C;Conte E;Cannone M;Caloiero R;Fonzino A;Tricarico D
神经生长因子（NGF）/脑源性神经营养因子（BDNF）和骨钙素具有调节能量、骨量、生殖和神经元功能的共同作用。为了研究NGF、BDNF和Osteocalcin之间的基因关系，我们通过RT-PCR比较了3月龄雌性和雄性小鼠脑、骨、白色/棕色脂肪组织（WAT/BAT）和生殖器官中Ngf、BDNF和Osteocalcin及其受体p75 NTR/NTRK 1、NTRK 2和Gprc 6a的转录水平。脑和骨分别用作NGF/BDNF和骨钙素的阳性对照。并对催产素（Oxt）及其受体（Oxtr）的作用进行了研究。与BDNF相比，Ngf表达显示相反的趋势。在两种性别中，BAT中的Ngf /p75 NTR表达比大脑高50%，但在骨中较低。与此相反，Bdnf在骨中的表达高于脑中，但在BAT/WAT中低。骨钙素基因在两性大鼠脑组织中均有表达，但Gprc 6a在脑组织和BAT/WAT中表达较低。正如预期的那样，Gprc 6a基因在骨中表达。在雄性和雌性中，Oxt基因在脑中表达显著，Oxtr基因在卵巢、脂肪和骨中表达显著。Ngf在生殖组织中高度表达，并且p75 NTR mRNA水平在睾丸/卵巢/子宫中分别比脑中高300、100和50%。相反，BDNF基因在生殖组织中不表达。正如预期的那样，Gprc 6a在睾丸中表达，但不在卵巢/子宫中表达。在脑、BAT和睾丸中发现了基因配体及其受体的表达水平之间的显著相关性，这表明在雄性和雌性中这些组织中不同基因的共同途径。骨钙素、Ngf或Bdnf基因表达水平的变化可能相互影响其他基因的表达水平。此外，不同的配体可能通过不同的受体亚型起作用。Oxt和Oxtr没有显示出显著的相关性。BAT中Ngf /p75 NTR的上调与NGF作为能量调节剂和BDNF调节骨一致。
Nerve Growth Factor (NGF)/Brain-derived Neurotrophic Factor (BDNF) and osteocalcin share common effects regulating energy, bone mass, reproduction and neuronal functions. To investigate on the gene-relationship between NGF, BDNF, and Osteocalcin we compared by RT-PCR the transcript levels of Ngf, Bdnf and Osteocalcin as well as of their receptors p75NTR/NTRK1, NTRK2, and Gprc6a in brain, bone, white/brown adipose tissue (WAT/BAT) and reproductive organs of 3 months old female and male mice. Brain and bone were used as positive controls for NGF/BDNF and Osteocalcin respectively. The role of oxitocin(Oxt) and its receptor(Oxtr) was also investigated. Ngf expression shows an opposite trend compared to Bdnf. Ngf /p75NTR expression is 50% higher in BAT than brain, in both genders, but lower in bone. In contrast, Bdnf expression in bone is higher than in brain, but low in BAT/WAT. We found Osteocalcin gene expressed in brain in both genders, but Gprc6a expression is low in brain and BAT/WAT. As expected, Gprc6a gene is expressed in bone. Oxt gene was markedly expressed in brain, Oxtr in the ovaries and in fat and bone in both genders. Ngf is highly expressed in reproductive tissues and p75NTR mRNA levels are respectively 300, 100, and 50% higher in testis/ovaries/uterus than in brain. In contrast, BDNF genes are not expressed in reproductive tissues. As expected, Gprc6a is expressed in testis but not in the ovaries/uterus. A significant correlation was found between the expression levels of the gene ligands and their receptors in brain, BAT and testis suggesting a common pathway of different genes in these tissues in either male and female. Changes in the expression levels of osteocalcin, Ngf, or Bdnf genes may mutually affect the expression levels of the others. Moreover, it may be possible that different ligands may operate through different receptor subtypes. Oxt and Oxtr failed to show significant correlation. The up-regulation of Ngf /p75NTR in BAT is consistent with NGF as an energy regulator and with BDNF regulating bone.
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影响因子：
64.5
作者：
Oury F;Khrimian L;Denny CA;Gardin A;Chamouni A;Goeden N;Huang YY;Lee H;Srinivas P;Gao XB;Suyama S;Langer T;Mann JJ;Horvath TL;Bonnin A;Karsenty G
通讯作者：
Karsenty G
影响因子：
18.2
作者：
通讯作者：
--
影响因子：
3.5
作者：
Wellendorph, Petrine;Johansen, Lars Dan;Braeuner-Osborne, Hans
通讯作者：
Braeuner-Osborne, Hans
影响因子：
2.3
作者：
Mueller, Dieter;Davidoff, Michail S.;Middendorff, Ralf
通讯作者：
Middendorff, Ralf
影响因子：
64.5
作者：
Lee, Na Kyung;Sowa, Hideaki;Karsenty, Gerard
通讯作者：
Karsenty, Gerard