Everything but the Kitchen Sink: An Analysis of Bacterial and Chemical Contaminants Found in Syringe Residue From People Who Inject Drugs.
Everything but the Kitchen Sink: An Analysis of Bacterial and Chemical Contaminants Found in Syringe Residue From People Who Inject Drugs.
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DOI：
10.1093/ofid/ofad628
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发表时间：
2024-01
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
作者：
注射吸毒者 (PWID) 面临严重伤口、侵袭性感染和吸毒过量的高风险。迄今为止，关于吸毒者在使用非法制造的芬太尼和兴奋剂时接触到的细菌和化学污染物的数据很少。减害组织历时 12 个月在密苏里州圣路易斯回收了以前使用过的注射吸毒设备。通过常规培养，然后对单个细菌分离株进行全基因组测序，分析注射器残留物中的细菌污染物。通过液相色谱-质谱法鉴定注射器残留物中的化学掺杂物。在分析的 160 个注射器中，有 58.75% 的注射器培养出了细菌。多种微生物的生长很常见，在 23.75% 的样品中观察到。蜡样芽孢杆菌是最常见的病原体，在 20.6% 的注射器残留物中观察到，紧随其后的是金黄色葡萄球菌，占 18.8%。对一百个注射器进行了质谱分析，结果表明化学掺杂物很常见，包括咖啡因、苯海拉明、利多卡因、奎宁和赛拉嗪。对废弃吸毒设备中注射器残留物的分析显示出化学和生物污染物，包括医学上重要的病原体和掺假物。对废弃吸毒设备的注射器残留物的分析表明，化学和生物污染物的流行率很高，包括医学上重要的病原体。
People who inject drugs (PWID) are at high risk of severe wounds, invasive infections, and overdoses. To date, there are few data on the bacterial and chemical contaminants PWID are exposed to when using illicitly manufactured fentanyls and stimulants. Previously used injection drug use equipment was recovered in St Louis, Missouri, by harm reduction organizations over a 12-month period. Syringe residue was analyzed for bacterial contaminants by routine culturing followed by whole genome sequencing of single bacterial isolates. Chemical adulterants in syringe residue were identified by liquid chromatography–mass spectrometry. Bacteria were cultured from 58.75% of 160 syringes analyzed. Polymicrobial growth was common and was observed in 23.75% of samples. Bacillus cereus was the most common pathogen present and was observed in 20.6% of syringe residues, followed closely by Staphylococcus aureus at 18.8%. One hundred syringes underwent mass spectrometry, which demonstrated that chemical adulterants were common and included caffeine, diphenhydramine, lidocaine, quinine, and xylazine. Analysis of syringe residue from discarded drug use equipment demonstrates both chemical and biological contaminants, including medically important pathogens and adulterants. Analysis of syringe residue of discarded drug use equipment demonstrates a high prevalence of both chemical and biological contaminants, including medically important pathogens.
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影响因子：
4.5
作者：
Fernandes RM;Cary M;Duarte G;Jesus G;Alarcão J;Torre C;Costa S;Costa J;Carneiro AV
通讯作者：
Carneiro AV
影响因子：
3
作者：
McLauchlin, J;Mithani, V;Ashton, JR
通讯作者：
Ashton, JR
影响因子：
2.2
作者：
Evans, Alexandra;Krause, Michael;Short, Luke C.
通讯作者：
Short, Luke C.
影响因子：
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作者：
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通讯作者：
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影响因子：
9.4
作者：
LEVIN, MH;WEINSTEIN, RA;KABINS, SA
通讯作者：
KABINS, SA