Isolation and functional characterization of a novel rice constitutive promoter
Isolation and functional characterization of a novel rice constitutive promoter
复制标题
新型水稻组成型启动子的分离和功能表征
DOI：
10.1007/s00299-014-1644-1
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发表时间：
2014-07
影响因子：
6.2
通讯作者：
Yang, Jian-Bo
中科院分区：
文献类型：
--
作者：
Li, Juan;Xu, Rong-Fang;Qin, Rui-Ying;Ma, Hui;Li, Hao;Zhang, Ying-Ping;Li, Li;Wei, Peng-Cheng;Yang, Jian-Bo
一个新的水稻构成启动子(POsCon1)被克隆。探讨了启动子活性的分子机制。POsCon1可作为农作物转基因工程的替代构成启动子，单子叶构成启动子是作物转基因工程的重要资源。在这篇报道中，我们从水稻基因OsDHAR1的5‘上游区域分离到一个新的启动子--Oscon1启动子(POsCon1)。在POsCon1：：GUS转基因水稻中，我们发现POsCon1在所有受试组织中都有广泛的表达谱。进一步分析了该启动子的表达情况，并与已鉴定的强组成型启动子进行了比较。POsCon1在大多数组织中表现出与OsCc1、OsAct1或ZmUbi启动子相当的活性，在根、种子和愈伤组织中的活性高于35S启动子。进一步的定量分析表明POsCon1活性不受发育阶段或环境因素的影响。进一步的5‘端缺失分析表明，不同的区域可能与POsCon1在不同组织中的强表达有关。总之，我们的结果表明POsCon1是一种新的组成型启动子，有可能用于转基因作物的开发。
Key messageA novel rice constitutive promoter (POsCon1) was isolated. The molecular mechanism of the promoter activity was investigated. POsCon1could be used as an alternative constitutive promoter for crop transgenic engineering.AbstractMonocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter,Oscon1promoter (POsCon1), from the 5′ upstream region of a constitutively expressed rice geneOsDHAR1. In POsCon1::GUStransgenic rice, we showed that POsCon1had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. POsCon1exhibited comparable activity toOsCc1,OsAct1orZmUbipromoters in most tissues, and more active than35Spromoter in roots, seeds, and calli. Further quantitative assays indicated that POsCon1activity was not affected by developmental stages or by environmental factors. Further, 5′-deletions analysis indicated that the distinct regions might contribute to the strong expression of POsCon1in different tissues. Overall, our results suggest that POsCon1is a novel constitutive promoter, which could potentially use in transgenic crop development.
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影响因子：
6.9
作者：
Park SH;Yi N;Kim YS;Jeong MH;Bang SW;Choi YD;Kim JK
通讯作者：
Kim JK
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
27.5
作者：
Zimmermann, Philip;Laule, Oliver;Gruissem, Wilhelm
通讯作者：
Gruissem, Wilhelm
影响因子：
6.2
作者：
Wang, J;Oard, JH
通讯作者：
Oard, JH