Analysis of five novel putative constitutive gene promoters in transgenic rice plants.
Analysis of five novel putative constitutive gene promoters in transgenic rice plants.
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DOI：
10.1093/jxb/erq076
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发表时间：
2010-05
影响因子：
6.9
通讯作者：
Kim JK
中科院分区：
文献类型：
--
作者：
Park SH;Yi N;Kim YS;Jeong MH;Bang SW;Choi YD;Kim JK
新型组成型基因启动子是作物生物技术的重要组成部分。我们的分析五个这样的启动子，APX，SCP 1，PGD 1，R1 G1 B，和EIF 5，在转基因水稻植株中报告。将这五个启动子区与gfp报告基因连接并转化到水稻中。使用荧光显微镜和q-RT-PCR，启动子活性进行了分析，与OsCc 1，Act 1和ZmUbi 1，以前被称为强组成型启动子比较。APX和PGD 1启动子在所有组织和阶段中指导高水平的基因表达，产生高达总可溶性蛋白1.3%的GFP。PGD 1在花和成熟的根中特别活跃。R1 G1 B在包括胚、胚乳和糊粉层的整个谷粒中是有活性的，因此代表了在之前未描述的整个种子中具有活性的组成型启动子。ZmUbi 1和R1 G1 B启动子在幼根和成熟叶中的活性明显降低，而APX、PGD 1、OsCc 1和Act 1启动子在营养和生殖组织中的活性都很高。总体而言，我们的研究结果表明，APX，PGD 1和R1 G1 B是新的基因启动子，在植物生长的所有阶段都具有不同的活性水平。
Novel constitutive gene promoters are essential components of crop biotechnology. Our analysis of five such promoters, APX, SCP1, PGD1, R1G1B, and EIF5, in transgenic rice plants is reported here. The five promoter regions were linked to the gfp reporter gene and transformed into rice. Using fluorescent microscopy and q-RT-PCR, promoter activities were analysed in comparison with OsCc1, Act1, and ZmUbi1, previously characterized as strong constitutive promoters. The APX and PGD1 promoters direct high levels of gene expression in all tissues and stages, producing GFP at levels of up to 1.3% of the total soluble protein. PGD1 is particularly active in flowers and mature roots. The R1G1B is active in the whole grain including the embryo, endosperm, and aleurone layer, and thus represents a constitutive promoter with activity in whole seeds that has not been described previously. The ZmUbi1 and R1G1B promoters are markedly less active in young roots and mature leaves whilst the APX, PGD1, OsCc1, and Act1 promoters are highly active in both vegetative and reproductive tissues. Overall, our results demonstrate that APX, PGD1, and R1G1B are novel gene promoters that are highly active at all stages of plant growth with distinct levels of activity.
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影响因子：
5.1
作者：
BATTRAW, MJ;HALL, TC
通讯作者：
HALL, TC
影响因子：
7.4
作者：
Oh, Se-Jun;Kim, Youn Shic;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
5.1
作者：
CORNEJO, MJ;LUTH, D;BLECHL, AE
通讯作者：
BLECHL, AE
DOI：
10.1007/bf00293832
发表时间：
1991-12-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
MCELROY, D;BLOWERS, AD;WU, R
通讯作者：
WU, R