Incorporation of phosphate group modulates bone cell attachment and differentiation on oligo(polyethylene glycol) fumarate hydrogel.
Incorporation of phosphate group modulates bone cell attachment and differentiation on oligo(polyethylene glycol) fumarate hydrogel.
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掺入磷酸盐组可调节在寡乙二醇（聚乙二醇）富马酸水凝胶上的骨细胞附着和分化。
DOI：
10.1016/j.actbio.2011.12.031
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发表时间：
2012-04
影响因子：
9.7
通讯作者：
Yaszemski, Michael J.
中科院分区：
文献类型：
--
作者：
Dadsetan, Mahrokh;Giuliani, Melissa;Wanivenhaus, Florian;Runge, M. Brett;Charlesworth, Jon E.;Yaszemski, Michael J.
在这项工作中，我们研究了一种合成水凝胶的发展，它含有一个带负电荷的磷酸基团，用作培养中骨细胞附着和分化的基质。将具有光反应性的含磷酸盐分子双（2-（甲基丙烯酰氧基）乙基）磷酸盐（BP）掺入低聚（聚乙二醇）富马酸盐水凝胶中，并对所得水凝胶的力学、流变学和热性能进行了表征。我们的研究结果表明，在水凝胶的压缩和存储模量与BP的掺入的变化。通过差示扫描量热法记录，改性也导致结晶度降低。我们的数据显示，BP的掺入以剂量依赖的方式改善了人胎儿成骨细胞（hFOB）的附着和分化。通过扫描电子显微镜和能量色散X射线分析验证的含磷酸盐表面的表面化学和矿化的变化被发现是重要的hFOB细胞附着和分化。我们还证明了含磷酸盐的水凝胶支持原代骨髓基质细胞的附着和分化。这些发现表明，BP改性水凝胶能够维持骨髓基质细胞和成骨细胞的附着和分化，这对骨再生至关重要。
In this work, we have investigated the development of a synthetic hydrogel that contains a negatively charged phosphate group for use as a substrate for bone cell attachment and differentiation in culture. The photoreactive, phosphate-containing molecule, bis(2-(methacryloyloxy)ethyl)phosphate (BP), was incorporated into oligo(polyethylene glycol) fumarate hydrogel and the mechanical, rheological and thermal properties of the resulting hydrogels were characterized. Our results showed changes in hydrogel compression and storage moduli with incorporation of BP. The modification also resulted in decreased crystallinity as recorded by differential scanning calorimetry. Our data revealed that incorporation of BP improved attachment and differentiation of human fetal osteoblast (hFOB) cells in a dose-dependent manner. A change in surface chemistry and mineralization of the phosphate-containing surfaces verified by scanning electron microscopy and energy dispersive X-ray analysis was found to be important for hFOB cell attachment and differentiation. We also demonstrated that phosphate-containing hydrogels support attachment and differentiation of primary bone marrow stromal cells. These findings suggest that BP-modified hydrogels are capable of sustaining attachment and differentiation of both bone marrow stromal cells and osteoblasts that are critical for bone regeneration.
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影响因子：
14
作者：
Wang, SF;Lu, LC;Yaszemski, MJ
通讯作者：
Yaszemski, MJ
影响因子：
14
作者：
You, Mingliang;Peng, Gongfeng;Chen, Guo-Qiang
通讯作者：
Chen, Guo-Qiang
影响因子：
5.3
作者：
Müller P;Bulnheim U;Diener A;Lüthen F;Teller M;Klinkenberg ED;Neumann HG;Nebe B;Liebold A;Steinhoff G;Rychly J
通讯作者：
Rychly J
影响因子：
6.2
作者：
Deshpande, Atul Suresh;Fang, Ping-An;Zhang, Xiaoyuan;Jayaraman, Thottala;Sfeir, Charles;Beniash, Elia
通讯作者：
Beniash, Elia
影响因子：
14
作者：
Burdick, JA;Ward, M;Langer, R
通讯作者：
Langer, R