Production of osteopontin by cultured porcine epithelial cell rests of Malassez.
Production of osteopontin by cultured porcine epithelial cell rests of Malassez.
复制标题
Malassez 培养的猪上皮细胞生产骨桥蛋白。
DOI：
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发表时间：
2005
影响因子：
3.5
通讯作者：
P. Bartold
中科院分区：
文献类型：
--
作者：
Julio C. Rincon;Yin Xiao;W. Young;P. Bartold
背景
Malassez 上皮细胞残骸 (ERM) 源自 Hertwig 上皮根鞘 (HERS)。在发育过程中，HERS 细胞在新形成的根表面沉积多种分子。需要研究 ERM 在根发育完成后保留这种潜力并分泌骨或牙骨质相关蛋白的可能性。本研究的目的是确定猪牙周组织上皮细胞剩余部分来源的细胞对非胶原蛋白骨桥蛋白 (OPN) 和骨唾液酸蛋白 (BSP) 的表达。
方法
ERM 和成纤维细胞是从猪牙周膜中培养的。使用透射电子显微镜、免疫组织化学、蛋白质印迹分析、逆转录聚合酶链反应以及在培养物中形成矿化结节的能力来鉴定和表征细胞。特别是研究了矿化组织相关蛋白 BSP 和 OPN 的表达。
结果
猪牙周膜细胞培养成功；分离并表征为上皮表型或成纤维细胞表型。尽管 ERM 在培养中没有形成矿化结节，但它们确实表达了大量 OPN 的 mRNA。
结论
这项研究的结果提供了 ERM 表达至少一种骨/牙骨质相关蛋白的 mRNA 的证据。这种功能是否与 ERM 在组织形成、炎症和再生中的作用一致仍有待确定。
BACKGROUND
The epithelial cell rests of Malassez (ERM) are derived from Hertwig's epithelial root sheath (HERS). During development the cells of HERS deposit a variety of molecules on the newly forming root surface. The possibility that ERM retain this potential after root development is completed and secrete bone or cementum-related proteins needs to be investigated. The purpose of this study was to determine the expression of the non-collagenous proteins osteopontin (OPN) and bone sialoprotein (BSP) by cells derived from the epithelial cell rests of porcine periodontium.
METHODS
ERM and fibroblasts were cultured from porcine periodontal ligament. The cells were identified and characterized using transmission electron microscopy, immunohistochemistry, western blot analysis of proteins, reverse transcription-polymerase chain reaction and ability to form mineralized nodules in culture. In particular the expression of the mineralized tissue-related proteins, BSP and OPN, was studied.
RESULTS
Cells from porcine periodontal ligaments were successfully cultured; separated and characterized as being of either an epithelial or fibroblastic phenotype. Although the ERM did not form mineralized nodules in culture, they did express a significant amount of mRNA for OPN.
CONCLUSION
The results from this study provide evidence that ERM express mRNA for at least one bone/cementum-related protein. Whether this function would be consistent with a role for ERM in tissue formation, inflammation and regeneration remains to be established.
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影响因子：
3.5
作者：
Harold C. Slavkin;P. Bringas;C. Bessem;V. Santos;Masanori Nakamura;Mingyoun Hsu;Malcolm L. Snead;M. Zeichner‐David;A. Fincham
通讯作者：
Harold C. Slavkin;P. Bringas;C. Bessem;V. Santos;Masanori Nakamura;Mingyoun Hsu;Malcolm L. Snead;M. Zeichner‐David;A. Fincham
影响因子：
4.3
作者：
D'Errico, JA;Berry, JE;Somerman, MJ
通讯作者：
Somerman, MJ
影响因子：
4.3
作者：
ARCEO, N;SAUK, JJ;SOMERMAN, MJ
通讯作者：
SOMERMAN, MJ
影响因子：
4.1
作者：
DErrico, JA;Macneil, RL;Somerman, MJ
通讯作者：
Somerman, MJ
使用 aIF、AE1 和 AE3 单克隆抗体对哺乳动物上皮角蛋白进行鉴定和分类。
DOI：
10.1111/j.1432-0436.1984.tb00263.x
发表时间：
1984
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Cooper,D;Schermer,A;Pruss,R;Sun,TT
通讯作者：
Sun,TT