Molecular characterization and functional analysis of DIGIRR from golden pompano (Trachinotus ovatus).
Molecular characterization and functional analysis of DIGIRR from golden pompano (Trachinotus ovatus).
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金鲳鱼 (Trachinotus ovatus) DIGIRR 的分子表征和功能分析
DOI：
10.3389/fimmu.2022.974310
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
哺乳动物单一免疫球蛋白(Ig)白介素1受体相关分子(SIGIRR)是Toll/IL-1受体(TIR)家族的重要成员，在炎症反应中起着重要的平衡作用。在本研究中，双Ig白介素1受体相关分子(DIGIRR)是SIGIRR的同源物，在金色的卵形尾巴(Trachinotus Ovatus)中被鉴定为trDIGIRR。TrDIGIRR的全长为2,167bp，开放阅读框为1,572bp，编码523个氨基酸。TrDIGIRR含有一个信号肽、两个Ig结构域、一个跨膜结构域和一个TIR结构域等几个保守结构域，与硬骨的同源序列具有很高的同源性。实时荧光定量聚合酶链式反应分析表明，trDIGIRR基因在所研究的所有组织中均有表达，其中在肠、肝和头肾组织中高表达。用溶藻弧菌、脂多糖和多聚肌苷多胞酸(PolyI：C)诱导trDIGIRR的表达。进一步分析表明，trDIGIRR主要定位于细胞质。免疫共沉淀法(co-IP)证实trDIGIRR能与髓系分化因子88(MyD88)相互作用，但不能与含有TIR结构域的诱导干扰素-β(TRIF)相互作用。我们的结果为研究鱼DIGIRR的免疫作用提供了依据。
Mammalian single immunoglobulin (Ig) interleukin-1 receptor related molecule (SIGIRR), an important member of the Toll/interleukin-1 receptor (TIR) family, plays important balancing roles in the inflammatory responses. In the present study, the double Ig interleukin-1 receptor related molecule (DIGIRR), the homologous of SIGIRR, was characterized in golden pompano (Trachinotus ovatus) (termed as trDIGIRR). The full-length cDNA of trDIGIRR was 2,167 bp with an open reading frame (ORF) of 1,572 bp encoding 523 amino acids. The trDIGIRR contained several conserved domains including a signal peptide, two Ig domains, a transmembrane domain and a TIR domain, and shared high sequence identities with its teleost counterparts. Realtime qPCR analysis revealed that the trDIGIRR was distributed in all tissues examined, with high expressions in intestine, liver and head kidney. The expressions of trDIGIRR were induced by Vibrio alginolyticus, lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly I:C) challenge. Further analysis revealed that trDIGIRR was mainly located in the cytoplasm. In addition, the co-immunoprecipitation (co-IP) assay identified that trDIGIRR could interact with myeloid differentiation factor 88 (MyD88), but not interact with TIR domain containing adaptor protein inducing interferon-β (TRIF). Our results provide basis for studying the immune role of fish DIGIRR.
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影响因子：
7.3
作者：
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影响因子：
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