The promoter architectural landscape of the Salmonella PhoP regulon.
The promoter architectural landscape of the Salmonella PhoP regulon.
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DOI：
10.1111/j.1365-2958.2012.08036.x
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发表时间：
2012-05
影响因子：
3.6
通讯作者：
Groisman EA
中科院分区：
文献类型：
--
作者：
Zwir I;Latifi T;Perez JC;Huang H;Groisman EA
DNA结合蛋白PhoP控制革兰氏阴性病原体肠道沙门氏菌血清型鼠伤寒沙门氏菌的毒力和Mg 2+稳态。PhoP调节大量基因的表达，这些基因在其祖先和编码产物的生化功能和生理作用方面都不同。这表明PhoP调节的基因差异表达。为了了解细菌激活剂如何产生不同的基因表达行为，我们研究了23个PhoP激活启动子中的顺式作用启动子特征（即PhoP结合位点的数量，以及它们相对于RNA聚合酶结合位点的方向和位置，以及构成PhoP结合位点的序列）。我们的研究结果表明，天然PhoP激活的启动子只利用有限数量的顺式作用功能或启动子架构的组合。我们确定，PhoP激活转录不同的机制，和祖先和水平收购PhoP激活基因有不同的启动子架构。
The DNA-binding protein PhoP controls virulence and Mg2+ homeostasis in the Gram-negative pathogen Salmonella enterica serovar Typhimurium. PhoP regulates expression of a large number of genes that differ both in their ancestry and in the biochemical functions and physiological roles of the encoded products. This suggests that PhoP-regulated genes are differentially expressed. To understand how a bacterial activator might generate varied gene expression behaviour, we investigated the cis-acting promoter features (i.e. the number of PhoP binding sites, as well as their orientation and location with respect to the sites bound by RNA polymerase and the sequences that constitute the PhoP binding sites) in 23 PhoP-activated promoters. Our results show that natural PhoP-activated promoters utilize only a limited number of combinations of cis-acting features – or promoter architectures. We determine that PhoP activates transcription by different mechanisms, and that ancestral and horizontally acquired PhoP-activated genes have distinct promoter architectures.
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影响因子：
64.5
作者：
GASTON, K;BELL, A;BUSBY, S
通讯作者：
BUSBY, S
影响因子：
5.7
作者：
Blanco, AG;Sola, M;Coll, M
通讯作者：
Coll, M
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3.7
作者：
Gal-Mor O;Elhadad D;Deng W;Rahav G;Finlay BB
通讯作者：
Finlay BB
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3.6
作者：
Feng, XH;Oropeza, R;Kenney, LJ
通讯作者：
Kenney, LJ
影响因子：
3.2
作者：
Aguirre, Andres;Cabeza, Maria Laura;Soncini, Fernando C.
通讯作者：
Soncini, Fernando C.