A SNP based high-density linkage map of Apis cerana reveals a high recombination rate similar to Apis mellifera.
A SNP based high-density linkage map of Apis cerana reveals a high recombination rate similar to Apis mellifera.
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DOI：
10.1371/journal.pone.0076459
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zeng ZJ
中科院分区：
文献类型：
--
作者：
Shi YY;Sun LX;Huang ZY;Wu XB;Zhu YQ;Zheng HJ;Zeng ZJ
东方蜜蜂，Apis cerana Fabricius，分布在亚洲南部和东部，从印度和中国到韩国和日本，东南到摩鹿加群岛。除蜜蜂外，该物种也广泛用于蜂蜜生产。中华蜜蜂也是研究社会行为、种姓决定、交配生物学、性选择和寄主-寄生虫相互作用的模式生物。可用于该物种分子研究的资源很少，并且从未构建过连锁图谱。连锁图谱是定量性状位点定位和基因组结构分析的前提。我们利用中国蜜蜂Apis cerana cerana构建了东方蜜蜂的首个连锁图谱。F2工人（N = 103）对126,990个单核苷酸多态性（SNPs）进行基因分型。在过滤掉低质量和未通过孟德尔检验的snp后，我们获得了3000个snp，其中1535个具有信息性并用于构建连锁图谱。初步图谱包含19个连锁群，并通过与棉蚜基因组的标记比对，将这19个连锁群定位到16条染色体上。最终的图谱包含16个连锁组，共有1535个标记。总遗传距离为3942.7 cM，最大连锁群（180个位点）为574.5 cM。16个连锁组中所有标记的平均标记间隔为2.6 cM。构建了包含1535个标记的中华蜜蜂高密度连锁图谱。由于该图谱是基于SNP标记的，因此与随机扩增的多态性DNA或在蜜蜂中使用的基于微卫星的图谱相比，它将使基因分型分析更加容易和快速。
The Eastern honey bee, Apis cerana Fabricius, is distributed in southern and eastern Asia, from India and China to Korea and Japan and southeast to the Moluccas. This species is also widely kept for honey production besides Apis mellifera. Apis cerana is also a model organism for studying social behavior, caste determination, mating biology, sexual selection, and host-parasite interactions. Few resources are available for molecular research in this species, and a linkage map was never constructed. A linkage map is a prerequisite for quantitative trait loci mapping and for analyzing genome structure. We used the Chinese honey bee, Apis cerana cerana to construct the first linkage map in the Eastern honey bee. F2 workers (N = 103) were genotyped for 126,990 single nucleotide polymorphisms (SNPs). After filtering low quality and those not passing the Mendel test, we obtained 3,000 SNPs, 1,535 of these were informative and used to construct a linkage map. The preliminary map contains 19 linkage groups, we then mapped the 19 linkage groups to 16 chromosomes by comparing the markers to the genome of A. mellfiera. The final map contains 16 linkage groups with a total of 1,535 markers. The total genetic distance is 3,942.7 centimorgans (cM) with the largest linkage group (180 loci) measuring 574.5 cM. Average marker interval for all markers across the 16 linkage groups is 2.6 cM. We constructed a high density linkage map for A. c. cerana with 1,535 markers. Because the map is based on SNP markers, it will enable easier and faster genotyping assays than randomly amplified polymorphic DNA or microsatellite based maps used in A. mellifera.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Arechavaleta-Velasco ME;Alcala-Escamilla K;Robles-Rios C;Tsuruda JM;Hunt GJ
通讯作者：
Hunt GJ
影响因子：
3.4
作者：
PENG, YS;FANG, YZ;GE, LS
通讯作者：
GE, LS
影响因子：
4.4
作者：
Aslam, Muhammad L.;Bastiaansen, John W. M.;Groenen, Martien A. M.
通讯作者：
Groenen, Martien A. M.
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
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