The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome.
The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome.
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Xis 的结构揭示了细丝形成的基础以及对分枝杆菌噬菌体嵌体内 DNA 弯曲的深入了解。
DOI：
10.1016/j.jmb.2013.10.002
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发表时间：
2014
影响因子：
5.6
通讯作者：
VanDemark,AndrewP
中科院分区：
文献类型：
--
作者：
Singh,Shweta;Plaks,JosephG;Homa,NicholasJ;Amrich,ChristopherG;Héroux,Annie;Hatfull,GrahamF;VanDemark,AndrewP
The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein–DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis crystals contain five subunits in the asymmetric unit, four of which align into a Xis filament and a fifth that is generated by an unusual domain swap. Extensive intersubunit contacts stabilize a bent filament-like arrangement with Xis monomers aligned head to tail. The structure implies a DNA bend of ~ 120°, which is in agreement with DNA bending measuredin vitro. Formation ofattR-containing intasomes requires only Int and Xis, distinguishing Pukovnik from lambda. Therefore, we conclude that, in Pukovnik, Xis-induced DNA bending is sufficient to promote intramolecular Int-mediated bridges during intasome formation.
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用于分析蛋白质诱导的 DNA 弯曲的质粒载体。
DOI：
10.1007/978-1-60327-015-1_32
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Zwieb,Christian;Adhya,Sankar
通讯作者：
Adhya,Sankar
影响因子：
14.9
作者：
Lewis, JA;Hatfull, GF
通讯作者：
Hatfull, GF
在 λ 位点特异性重组中交换 DNA 链并感知同源性，无需分支迁移
影响因子：
9.2
作者：
S. Nunes;M. Azaro;A. Landy
通讯作者：
A. Landy
影响因子：
64.5
作者：
Rice, PA;Yang, SW;NAsh, HA
通讯作者：
NAsh, HA
影响因子：
5.7
作者：
Ellenberger, T;Landy, A
通讯作者：
Landy, A