Rho kinase activity controls directional cell movements during primitive streak formation in the rabbit embryo.
Rho kinase activity controls directional cell movements during primitive streak formation in the rabbit embryo.
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DOI：
10.1242/dev.111583
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Viebahn C
中科院分区：
文献类型：
--
作者：
Stankova V;Tsikolia N;Viebahn C
在动物原肠胚形成过程中，胚胎轴的形成伴随着上皮-间质转化（EMT），这是受精后细胞形状的第一个主要变化。EMT发生在不同的地形安排中，比如两栖动物的圆形胚孔，鸟类和哺乳动物的直原始条纹，或者其他羊膜动物（如爬行动物）的中间原肠胚形成形式。平面细胞运动是安排特定原肠胚形成模式的主要候选，但它们在不同脊椎动物类别中的作用尚未达成共识。在这里，我们测试了干扰Rho激酶介导的细胞运动对兔胚泡原肠胚地形的影响，兔胚泡具有大多数哺乳动物典型的扁平胚盘。延时视频显微镜、电子显微镜、基因表达和形态计量学分析表明，抑制ROCK活性的影响-除了正常的组织者区域-原始条纹形成的剂量依赖性中断；这种破坏导致了圆形、弧形或中间形状，让人想起两栖动物、鱼类和爬行动物身上发现的那些。我们的研究结果揭示了岩石控制的定向细胞运动在兔原始条纹形成过程中的关键作用，并强调了细胞运动的时空调节对原肠胚形式进化的可能性。摘要：ROCK调节兔原肠胚细胞的运动、极化、嵌入和分裂，揭示了Wnt-PCP信号在原始条纹形成中的作用。
During animal gastrulation, the specification of the embryonic axes is accompanied by epithelio-mesenchymal transition (EMT), the first major change in cell shape after fertilization. EMT takes place in disparate topographical arrangements, such as the circular blastopore of amphibians, the straight primitive streak of birds and mammals or in intermediate gastrulation forms of other amniotes such as reptiles. Planar cell movements are prime candidates to arrange specific modes of gastrulation but there is no consensus view on their role in different vertebrate classes. Here, we test the impact of interfering with Rho kinase-mediated cell movements on gastrulation topography in blastocysts of the rabbit, which has a flat embryonic disc typical for most mammals. Time-lapse video microscopy, electron microscopy, gene expression and morphometric analyses of the effect of inhibiting ROCK activity showed – besides normal specification of the organizer region – a dose-dependent disruption of primitive streak formation; this disruption resulted in circular, arc-shaped or intermediate forms, reminiscent of those found in amphibians, fishes and reptiles. Our results reveal a crucial role of ROCK-controlled directional cell movements during rabbit primitive streak formation and highlight the possibility that temporal and spatial modulation of cell movements were instrumental for the evolution of gastrulation forms. Summary: ROCK regulates cell motility, polarisation, intercalation and division in gastrulating rabbit embryos, revealing a role for Wnt-PCP signalling in primitive streak formation.
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影响因子：
3.7
作者：
Coolen M;Nicolle D;Plouhinec JL;Gombault A;Sauka-Spengler T;Menuet A;Pieau C;Mazan S
通讯作者：
Mazan S
影响因子：
2.4
作者：
Hue, I;Renard, JP;Viebahn, C
通讯作者：
Viebahn, C
DOI：
10.1002/jez.b.00013
发表时间：
2003-04-15
影响因子：
2.2
作者：
Richardson, MK;Chipman, AD
通讯作者：
Chipman, AD
影响因子：
9.2
作者：
Fischer, A;Viebahn, C;Blum, M
通讯作者：
Blum, M
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF