Electrostatic Variation of Haemagglutinin as a Hallmark of the Evolution of Avian Influenza Viruses.
Electrostatic Variation of Haemagglutinin as a Hallmark of the Evolution of Avian Influenza Viruses.
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DOI：
10.1038/s41598-018-20225-3
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发表时间：
2018-01-31
影响因子：
4.6
通讯作者：
Filippini F
中科院分区：
文献类型：
--
作者：
Heidari A;Righetto I;Filippini F
禽流感病毒是一种严重影响公众健康和家禽业的人畜共患病原体。监测病毒进化和传播对监测和追踪规划至关重要，目前这些规划基于血清学或DNA测序-系统发育分析。然而，病毒与宿主的相互作用、抗原漂移和病毒分支的传播强烈依赖于衣壳蛋白表面特征的变化。我们在这里报告，在硅比较结构分析血凝素可以揭示相关的进化指纹，特别是当与基于序列的分析集成。用不同的方法对来自野生鸟类和家禽的H9病毒株进行系统发育分析，可靠地将病毒聚类为五个主要组。随后的结构特征比较表明，这种聚类和表面静电指纹之间是一致的。这些后一种指纹将静电荷和等等高线的群体特异性变化与已知的参与免疫逃逸和宿主特异性调节的血凝素位点联系起来。这项工作表明，结构和序列比较的整合可能会加强对病毒进化趋势和相关机制的研究。
Avian influenza virus is a zoonotic agent that significantly impacts public health and the poultry industry. Monitoring viral evolution and spread is crucial for surveillance and tracing programmes, which are currently based on serological or DNA sequencing-phylogenetics analysis. However, virus-host interactions, antigenic drift and spreading of viral clades strongly depend on variation in the surface features of capsid proteins. We report here that in silico comparative structural analysis of haemagglutinin can reveal relevant evolutionary fingerprints, particularly when integrated with sequence-based analyses. Phylogenetic analyses of H9 viral strains from wild birds and poultry, performed with different methods, reliably led to clustering of viruses into five main groups. Subsequent comparison of structural features showed congruence between such clustering and surface electrostatic fingerprints. These latter fingerprints relate group-specific variations in electrostatic charges and isocontours to well-known haemagglutinin sites involved in the modulation of immune escape and host specificity. This work suggests that the integration of structural and sequence comparisons may enhance investigations of trends and relevant mechanisms in viral evolution.
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