Cryptic Species Diversification of the Pedicularis siphonantha Complex (Orobanchaceae) in the Mountains of Southwest China Since the Pliocene.
Cryptic Species Diversification of the Pedicularis siphonantha Complex (Orobanchaceae) in the Mountains of Southwest China Since the Pliocene.
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上新世以来中国西南山区马先蒿杂丛（列当科）的隐秘物种多样性
DOI：
10.3389/fpls.2022.811206
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发表时间：
2022
影响因子：
5.6
通讯作者：
Yu WB
中科院分区：
文献类型：
--
作者：
Liu R;Wang H;Yang JB;Corlett RT;Randle CP;Li DZ;Yu WB
形态学的方法往往无法界定最近衍生的物种复合体中的物种。这可能在历史收藏中加剧，这些收藏可能在标本制备和保存中失去了关键特征。在这里，我们研究了中国西南山区特有的管花马先蒿复合体。这个复合体的特点是它的红色/紫色/粉红色和长管状花冠，和扭曲，具喙的盔瓣。然而，植物标本馆的标本往往很难确定的物种。利用nrITS或核核糖体内转录间隔区（nrITS）+质体DNA（ptDNA）的分子方法已成功地用于马先蒿属的种鉴定。为了解决在管花马先蒿复杂的分类混乱，我们重建nrITS和四个质体DNA位点（matK，rbcL，trnH-psbA，和trnL-F）的复杂的遗传。为了尽可能多地恢复系统发育史，我们在种群水平上对个体进行了采样。nrITS和ptDNA数据集之间的拓扑不一致恢复的分支，包括两个广泛分布的物种，马先蒿milliana和马先蒿tenuituba。基于形态学、地理学和遗传学证据，我们认为丽江玉龙雪山马先蒿与马先蒿/马先蒿sp.1之间以及宁蒗细管马先蒿与细管马先蒿之间存在杂交/渐渗现象。在去除长管马先蒿（Pedicularis dolichosiphon，nrITS）和milliana马先蒿（P. milliana，ptDNA）中相互冲突的DNA区域后，nrITS和ptDNA的串联序列可区分出管花马先蒿复合体中的11个种，其中包括2个未描述的种，分别来自交子雪山和玉龙雪山。系统地理学分析表明，该复合体起源于横断山脉以南，向北扩展至喜马拉雅山脉和云贵高原。此外，青藏高原的隆升和气候振荡可能推动了该综合体的进一步多样化。
Morphological approaches often fail to delimit species in recently derived species complexes. This can be exacerbated in historical collections which may have lost key features in specimen preparation and preservation. Here, we examine the Pedicularis siphonantha complex, endemic to the Mountains of Southwest China. This complex is characterized by its red/purple/pink and long-tubular corolla, and twisted, beaked galea. However, herbarium specimens are often difficult to identify to species. Molecular approaches using nrITS or nuclear ribosomal internal transcribed spacer (nrITS) + plastid DNA (ptDNA) have been successfully used for species identification in Pedicularis. To resolve taxonomic confusion in the Pedicularis siphonantha complex, we reconstructed phylogenies of the complex using nrITS and four plastid DNA loci (matK, rbcL, trnH-psbA, and trnL-F). To recover as much of the phylogenetic history as possible, we sampled individuals at the population level. Topological incongruence between the nrITS and ptDNA datasets was recovered in clades including two widely distributed species, Pedicularis milliana and Pedicularis tenuituba. Based on morphological, geographical, and genetic evidence, we suggest that hybridization/introgression has occurred between P. milliana and Pedicularis sigmoidea/Pedicularis sp. 1 in the Yulong Snow Mountain of Lijiang, northwest Yunnan, and between P. tenuituba and Pedicularis leptosiphon in Ninglang, northwest Yunnan. After removing conflicting DNA regions in Pedicularis dolichosiphon (nrITS) and P. milliana (ptDNA), the concatenated nrITS and ptDNA phylogenies distinguish 11 species in the P. siphonantha complex, including two undescribed species, from the Jiaozi and Yulong Snow Mountains, respectively. Phylogeographical analyses indicate that the P. siponantha complex originated from south of the Hengduan Mountains, expanding north to the Himalayas and the Yunnan-Guizhou Plateau. Moreover, the uplift of the Qinghai-Tibet Plateau and climate oscillations may have driven further diversification in the complex.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
3.4
作者：
Bouckaert RR;Drummond AJ
通讯作者：
Drummond AJ
影响因子：
10.7
作者：
Darriba, Diego;Posada, David;Flouri, Tomas
通讯作者：
Flouri, Tomas
影响因子：
10
作者：
Favre, Adrien;Paeckert, Martin;Muellner-Riehl, Alexandra N.
通讯作者：
Muellner-Riehl, Alexandra N.