Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae).
Comparative Analysis of Complete Chloroplast Genomes of Anemoclema, Anemone, Pulsatilla, and Hepatica Revealing Structural Variations Among Genera in Tribe Anemoneae (Ranunculaceae).
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对银莲花、银莲花、白头翁和肝花的完整叶绿体基因组的比较分析揭示了银莲花族（毛茛科）属之间的结构变异
DOI：
10.3389/fpls.2018.01097
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发表时间：
2018
影响因子：
5.6
通讯作者：
Xie L
中科院分区：
文献类型：
--
作者：
Liu H;He J;Ding C;Lyu R;Pei L;Cheng J;Xie L
银莲花属植物叶绿体基因组的结构重排已经基于限制性酶切位点的遗传作图而被报道，但从未被基因组研究证实。我们使用了新一代测序方法来表征完整的叶绿体基因组的五个物种的部落Anemoneae。使用从头组装方法结合常规桑格测序来组装质体基因组以填补空白。比较了银莲花族与毛茛科其它植物叶绿体基因组的基因顺序。在银莲花族中检测到多个倒位和易位。与毛茛科其他属相比，银莲花属、银莲花属、肝属和白头翁属具有相同的基因顺序，在大的单拷贝区（LSC）中包含三个倒位。Archiclematis，铁线莲，Naravelia共享相同的基因顺序，包含两个倒位和一个转座LSC。一个大约4.4 kb的扩增区反向重复序列（IR）的区域中检测到的部落海葵，这表明该扩展事件可能是一个突触。使用简约性和贝叶斯方法与分区模型的实施产生了一个很好的解决毛茛科植物的基因组分析。这些结果表明，叶绿体基因组的评价可能会导致提高分辨率的家庭progenies。样品银莲花，肝，白头翁进行了测试，形成并系的等级内部落银莲花。海葵属是铁线莲属的姐妹分支。银莲花族质体基因组的结构变异为毛茛科的系统发育提供了有力的信息。
Structural rearrangements of Anemone species' chloroplast genome has been reported based on genetic mapping of restriction sites but has never been confirmed by genomic studies. We used a next-generation sequencing method to characterize the complete chloroplast genomes of five species in the tribe Anemoneae. Plastid genomes were assembled using de novo assembling methods combined with conventional Sanger sequencing to fill the gaps. The gene order of the chloroplast genomes of tribe Anemoneae was compared with that of other Ranunculaceae species. Multiple inversions and transpositions were detected in tribe Anemoneae. Anemoclema, Anemone, Hepatica, and Pulsatilla shared the same gene order, which contained three inversions in the large single copy region (LSC) compared to other Ranunculaceae genera. Archiclematis, Clematis, and Naravelia shared the same gene order containing two inversions and one transposition in LSC. A roughly 4.4 kb expansion region in inverted repeat (IR) regions was detected in tribe Anemoneae, suggesting that this expansion event may be a synapomorphy for this group. Plastome phylogenomic analyses using parsimony and a Bayesian method with implementation of partitioned models generated a well resolved phylogeny of Ranunculaceae. These results suggest that evaluation of chloroplast genomes may result in improved resolution of family phylogenies. Samples of Anemone, Hepatica, and Pulsatilla were tested to form paraphyletic grades within tribe Anemoneae. Anemoclema was a sister clade to Clematis. Structual variation of the plastid genome within tribe Anemoneae provided strong phylogenetic information for Ranunculaceae.
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DOI：
10.1007/bf02464880
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
3.6
作者：
Huang DI;Cronk QC
通讯作者：
Cronk QC
影响因子：
4.1
作者：
Jabbour, Florian;Renner, Susanne S.
通讯作者：
Renner, Susanne S.
影响因子：
30.8
作者：
GISH, W;STATES, DJ
通讯作者：
STATES, DJ
影响因子：
4.1
作者：
Doyle, JJ;Doyle, JL;Palmer, JD
通讯作者：
Palmer, JD