Ultrasonic Microbubble Cavitation Deliver Gal-3 shRNA to Inhibit Myocardial Fibrosis after Myocardial Infarction.
Ultrasonic Microbubble Cavitation Deliver Gal-3 shRNA to Inhibit Myocardial Fibrosis after Myocardial Infarction.
复制标题
超声微泡空化传递 Gal-3 shRNA 抑制心肌梗死后心肌纤维化
DOI：
10.3390/pharmaceutics15030729
复制
发表时间：
2023-02-22
期刊：
影响因子：
5.4
通讯作者：
Xie M
中科院分区：
文献类型：
--
作者：
Li W;Jin Q;Zhang L;He S;Song Y;Xu L;Deng C;Wang L;Qin X;Xie M
半乳糖凝集素-3（Galectin-3，Gal-3）以多种方式参与心肌纤维化（myocardial fibrosis，MF）。抑制Gal-3的表达可以有效地干预MF。本研究旨在探讨超声靶向微泡破坏（UTMD）介导Gal-3短发夹RNA（shRNA）转染抗心肌纤维化的价值及其机制。建立大鼠心肌梗死（MI）模型，随机分为对照组和Gal-3 shRNA/CMBs+超声组（Gal-3 shRNA/CMBs + US）。超声心动图每周测量左心室射血分数（LVEF），并收获心脏以分析纤维化、Gal-3和胶原蛋白表达。与对照组相比，Gal-3 shRNA/CMB + US组的LVEF有所改善。第21天，Gal-3 shRNA/CMB + US组心肌Gal-3表达降低。Gal-3 shRNA/CMB + US组心肌纤维化面积比对照组减少6.9 ± 0.41%。在抑制Gal-3后，胶原蛋白（胶原蛋白I和III）的产生下调，并且Col I/Col III的比率降低。结论：UTMD介导的Gal-3 shRNA转染能有效沉默心肌组织Gal-3的表达，减轻心肌纤维化，保护心脏射血功能。
Galectin-3 (Gal-3) participates in myocardial fibrosis (MF) in a variety of ways. Inhibiting the expression of Gal-3 can effectively interfere with MF. This study aimed to explore the value of Gal-3 short hairpin RNA (shRNA) transfection mediated by ultrasound-targeted microbubble destruction (UTMD) in anti-myocardial fibrosis and its mechanism. A rat model of myocardial infarction (MI) was established and randomly divided into control and Gal-3 shRNA/cationic microbubbles + ultrasound (Gal-3 shRNA/CMBs + US) groups. Echocardiography measured the left ventricular ejection fraction (LVEF) weekly, and the heart was harvested to analyze fibrosis, Gal-3, and collagen expression. LVEF in the Gal-3 shRNA/CMB + US group was improved compared with the control group. On day 21, the myocardial Gal-3 expression decreased in the Gal-3 shRNA/CMBs + US group. Furthermore, the proportion of the myocardial fibrosis area in the Gal-3 shRNA/CMBs + US group was 6.9 ± 0.41% lower than in the control group. After inhibition of Gal-3, there was a downregulation in collagen production (collagen I and III), and the ratio of Col I/Col III decreased. In conclusion, UTMD-mediated Gal-3 shRNA transfection can effectively silence the expression of Gal-3 in myocardial tissue, reduce myocardial fibrosis, and protect the cardiac ejection function.
登录
查看更多内容
心力衰竭和心血管疾病中 Galectin-3 的激活和抑制：最新进展。
影响因子：
12.4
作者：
Suthahar N;Meijers WC;Silljé HHW;Ho JE;Liu FT;de Boer RA
通讯作者：
de Boer RA
影响因子：
16.1
作者：
Liu M;López de Juan Abad B;Cheng K
通讯作者：
Cheng K
影响因子：
5.6
作者：
Blanda V;Bracale UM;Di Taranto MD;Fortunato G
通讯作者：
Fortunato G
影响因子：
4
作者：
Li, Sha;Li, Shuren;Deng, Wenhao
通讯作者：
Deng, Wenhao
影响因子：
20.1
作者：
Travers JG;Kamal FA;Robbins J;Yutzey KE;Blaxall BC
通讯作者：
Blaxall BC