Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars.
Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars.
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DOI：
10.3389/fpls.2016.01307
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发表时间：
2016
影响因子：
5.6
通讯作者：
Dai S
中科院分区：
文献类型：
--
作者：
Jin X;Huang H;Wang L;Sun Y;Dai S
花青素（cyanidin，Cy）、天竺葵素（pelargonidin，Pg）和飞燕草素（delphinidin，Dp）是三条主要的花青素苷生物合成途径，它们调节类黄酮代谢流，并分别负责红色、橙子和蓝色花的颜色。不同的物种已经进化出形成分支途径的多种调节机制。以5个不同花色的瓜迪奥拉品种为试材，研究了不同花色瓜迪奥拉品种的花色差异。我们发现，白色和黄色品种不积累花青素，蓝色，粉红色和胭脂红品种主要积累不同密度的Dp，Pg和Cy。随后的转录组分析确定，有43个单一基因编码的花青素生物合成基因的蓝色品种。我们还结合了化学和转录组学分析，以研究与所观察到的S系列花色素差异相关的主要代谢途径。cruentus。结果表明，在白色和黄色品种中，ScbHLH 17和ScCHI 1/2编码区的突变导致花青素的形成消失; ScF 3 ′H1、ScF 3 ′5′H和ScDFR 1/2基因对柚皮素的竞争决定了Cy、Dp和Pg途径分支代谢通量的差异。本研究为花色苷分支调控提供了新的思路，同时也为花色修饰提供了新的基因资源（包括ScF 3 ′5 ′H、ScF 3 ′H和ScDFR）。
The cyanidin (Cy), pelargonidin (Pg), and delphinidin (Dp) pathways are the three major branching anthocyanin biosynthesis pathways that regulate flavonoid metabolic flux and are responsible for red, orange, and blue flower colors, respectively. Different species have evolved to develop multiple regulation mechanisms that form the branched pathways. In the current study, five Senecio cruentus cultivars with different colors were investigated. We found that the white and yellow cultivars do not accumulate anthocyanin and that the blue, pink, and carmine cultivars mainly accumulate Dp, Pg, and Cy in differing densities. Subsequent transcriptome analysis determined that there were 43 unigenes encoding anthocyanin biosynthesis genes in the blue cultivar. We also combined chemical and transcriptomic analyses to investigate the major metabolic pathways that are related to the observed differences in flower pigmentation in the series of S. cruentus. The results showed that mutations of the ScbHLH17 and ScCHI1/2 coding regions abolish anthocyanin formation in the white and the yellow cultivars; the competition of the ScF3′H1, ScF3′5′H, and ScDFR1/2 genes for naringenin determines the differences in branching metabolic flux of the Cy, Dp, and Pg pathways. Our findings provide new insights into the regulation of anthocyanin branching and also supplement gene resources (including ScF3′5 ′H, ScF3′H, and ScDFRs) for flower color modification of ornamentals.
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