TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum.
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum.
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TLR7 调节感染日本血吸虫的 C57BL/6 小鼠脾脏中的 B 细胞免疫反应
DOI：
10.1371/journal.pntd.0009943
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发表时间：
2021-11
影响因子：
3.8
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Wei H;Xie H;Qu J;Xie A;Xie S;Huang H;Li J;Fang C;Shi F;Qiu H;Qi Y;Tian X;Yang Q;Huang J
B细胞在血吸虫感染诱导的疾病中起重要作用。Toll样受体7（TLR7）是先天性免疫受体的一种胞内成员。TLR7在B细胞介导的免疫应答中的作用仍不清楚。在此，C57BL/6小鼠经皮肤感染日本血吸虫5 - 6周。感染小鼠中B细胞的百分比和数量增加（p < 0.05），并且B细胞上许多活化及功能相关分子也发生了改变。感染小鼠更多的脾细胞表达TLR7，且B细胞是主要的表达细胞群。此外，与感染的野生型（WT）小鼠相比，日本血吸虫感染的Toll样受体7基因敲除（TLR7 KO）小鼠脾B细胞表面的可溶性虫卵抗原（SEA）特异性抗体水平更低，活化相关分子也更少（p < 0.05）。另外，SEA诱导野生型幼稚小鼠B细胞活化的能力比诱导TLR7 KO小鼠的能力略高（p < 0.05）。最后，TLR7对B细胞的作用依赖于核因子 - κB p65的活化。总之，发现TLR7可调节日本血吸虫感染的C57BL/6小鼠的脾B细胞应答。
血吸虫病严重危害热带和亚热带地区的公共卫生和社会发展。B细胞在血吸虫感染诱导的疾病中起重要作用。TLR7是先天性免疫受体的一种胞内成员。TLR7在B细胞介导的免疫应答中的作用仍不清楚。在此，我们发现感染小鼠中B细胞的百分比和数量增加（p < 0.05），并且B细胞上许多活化及功能相关分子的表达也发生了改变。B细胞是表达TLR7的主要细胞群。当TLR7基因被敲除时，日本血吸虫感染小鼠中可溶性虫卵抗原（SEA）特异性抗体和活化相关分子减少。此外，SEA可通过TLR7诱导B细胞活化。最后，我们发现TLR7对B细胞的作用依赖于核因子 - κB p65的活化。在本研究中，探究了日本血吸虫感染的C57BL/6小鼠脾脏中B细胞的特性，并研究了TLR7在B细胞活化和分化过程中的作用。
B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Schistosomiasis seriously jeopardizes public health and social development in tropical and subtropical regions. B cells play an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, we found the percentage and numbers of B cells increased in the infected mice (p < 0.05), and the expression of many activation and function associated molecules were also changed on B cells. B cells were served as the main cell population expressed TLR7. When TLR7 gene was knockout, the soluble egg antigen (SEA) specific antibody and activation associated molecules were decreased in S. japonicum infected mice. Additionally, SEA could induce the activation of B cells by TLR7. Finally, we found the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. In this study, the characteristic of B cells in the spleen of S. japonicum infected C57BL/6 mice was explored, and the role of TLR7 on the progress of B cell activation and differentiation was investigated.
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影响因子：
3.7
作者：
Ji F;Liu Z;Cao J;Li N;Liu Z;Zuo J;Chen Y;Wang X;Sun J
通讯作者：
Sun J
影响因子：
12.8
作者：
Hartwell BL;Pickens CJ;Leon M;Northrup L;Christopher MA;Griffin JD;Martinez-Becerra F;Berkland C
通讯作者：
Berkland C
影响因子：
4.4
作者：
Hoyer, Bimba F.;Radbruch, Andreas
通讯作者：
Radbruch, Andreas
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影响因子：
4
作者：
Duan, Yinong;Gu, Xijuan;He, Xingxin
通讯作者：
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影响因子：
8.1
作者：
Colley DG;Andros TS;Campbell CH Jr
通讯作者：
Campbell CH Jr