An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes.
An important step towards a prevascularized islet microencapsulation device: in vivo prevascularization by combination of mesenchymal stem cells on micropatterned membranes.
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DOI：
10.1007/s10856-018-6178-6
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发表时间：
2018-11-09
期刊：
影响因子：
--
通讯作者：
van Apeldoorn A
中科院分区：
文献类型：
--
作者：
Groot Nibbelink M;Skrzypek K;Karbaat L;Both S;Plass J;Klomphaar B;van Lente J;Henke S;Karperien M;Stamatialis D;van Apeldoorn A
胰岛肝外移植可提高胰岛移植后的存活率。当胰岛被输入肝脏时，60-70%的胰岛在移植后立即丢失。肝外移植成功的一个重要因素是移植物周围血管化良好的组织。已知有许多用于增强血管形成的策略，例如添加具有内皮潜能的细胞、与血管生成因子的组合和/或在装置的暴露表面处应用表面形貌。以前，我们开发了多孔的，微图案化的膜，可以作为胰岛封装装置的盖子，我们表明，表面形貌诱导人脐静脉内皮细胞（HUVEC）的对齐和互连。这是在不添加水凝胶的情况下实现的，水凝胶通常用于血管生成测定。在这项工作中，我们通过将这种微图案盖子与间充质干细胞（MSC）结合以促进体内预血管化，进一步实现了该装置的临床应用。对于HUVEC，微图案化膜在体外诱导MSC排列和组织，这是血管形成的重要贡献者，而在体内（皮下大鼠模型），它们有助于改善植入物预血管化。事实上，在微图案化膜上接种的MSC的组合在80%的切片中诱导了最高的血管形成评分。
Extrahepatic transplantation of islets of Langerhans could aid in better survival of islets after transplantation. When islets are transfused into the liver 60-70% of them are lost immediately after transplantation. An important factor for a successful extrahepatic transplantation is a well-vascularized tissue surrounding the implant. There are many strategies known for enhancing vessel formation such as adding cells with endothelial potential, the combination with angiogenic factors and / or applying surface topography at the exposed surface of the device. Previously we developed porous, micropatterned membranes which can be applied as a lid for an islet encapsulation device and we showed that the surface topography induces human umbilical vein endothelial cell (HUVEC) alignment and interconnection. This was achieved without the addition of hydrogels, often used in angiogenesis assays. In this work, we went one step further towards clinical implementation of the device by combining this micropatterned lid with Mesenchymal Stem Cells (MSCs) to facilitate prevascularization in vivo. As for HUVECs, the micropatterned membranes induced MSC alignment and organization in vitro, an important contributor to vessel formation, whereas in vivo (subcutaneous rat model) they contributed to improved implant prevascularization. In fact, the combination of MSCs seeded on the micropatterned membrane induced the highest vessel formation score in 80% of the sections.
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影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
14
作者：
Heilshorn, SC;DiZio, KA;Tirrell, DA
通讯作者：
Tirrell, DA
影响因子：
4.2
作者：
Grundfest-Broniatowski, Sharon F.;Tellioglu, Gurkan;Kennedy, Joseph P.
通讯作者：
Kennedy, Joseph P.
影响因子：
7.7
作者：
Harlan DM;Kenyon NS;Korsgren O;Roep BO;Immunology of Diabetes Society
通讯作者：
Immunology of Diabetes Society
影响因子：
3.8
作者：
Anderson, Deirdre E. J.;Hinds, Monica T.
通讯作者：
Hinds, Monica T.