Preparation of penta-O-galloyl-β-D-glucose from tannic acid and plasma pharmacokinetic analyses by liquid-liquid extraction and reverse-phase HPLC.
Preparation of penta-O-galloyl-β-D-glucose from tannic acid and plasma pharmacokinetic analyses by liquid-liquid extraction and reverse-phase HPLC.
复制标题
DOI：
10.1016/j.jpba.2010.09.028
复制
发表时间：
2011-02-20
影响因子：
3.4
通讯作者：
Lü J
中科院分区：
文献类型：
--
作者：
Li L;Shaik AA;Zhang J;Nhkata K;Wang L;Zhang Y;Xing C;Kim SH;Lü J
The gallotannin penta-O-galloyl-beta-D-glucose (PGG) has many biological activities including in vivo anti-cancer efficacy. We present in this paper a scaled-up protocol for its preparation in high purity from tannic acid by acidic methanolysis with typical yield of 15%. We also describe a method for the analysis of PGG in mouse plasma by HPLC and its application in preliminary pharmacokinetic studies. A liquid–liquid extraction (LLE) protocol was optimized for the extraction of PGG from mouse plasma. The extraction efficiency for PGG at 1 μg/mL in mouse plasma was 70.0 ± 1.3% (n=5). The limit of detection (LOD) for PGG was approximately 0.2 μg/mL. Preliminary pharmacokinetic parameters of PGG following a single i.p. injection with 5% ethanol/saline vehicle in mice were established. The peak plasma PGG concentrations (Cmax) were approximately 3–4 μM at a dose of 0.5 mg per mouse (~20 mg/kg) at 2 h post-injection (Tmax).
登录
查看更多内容
影响因子：
4.4
作者：
Akkina, Sanjeev K.;Zhang, Yan;Ibrahim, Hassan N.
通讯作者：
Ibrahim, Hassan N.
影响因子：
6.1
作者：
Hartzfeld, PW;Forkner, R;Hagerman, AE
通讯作者：
Hagerman, AE
影响因子：
5.6
作者：
Cavalher-Machado, Simone Campos;Rosas, Elaine Cruz;Mueller de Oliveira Henriques, Maria das Gracas
通讯作者：
Mueller de Oliveira Henriques, Maria das Gracas
影响因子：
6.1
作者：
Kuo, Po-Tsun;Lin, Tsung-Pang;Way, Tzong-Der
通讯作者：
Way, Tzong-Der
影响因子：
2
作者：
Lee, Sung-Jin;Lee, Hak-Kyo;Mar, Woongchon
通讯作者：
Mar, Woongchon