Genetics and immunity in the era of single-cell genomics.
Genetics and immunity in the era of single-cell genomics.
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DOI：
10.1093/hmg/ddw192
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发表时间：
2016-10-01
影响因子：
3.5
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Vieira Braga FA;Teichmann SA;Chen X
单细胞基因组学（SCG）领域的最新发展正在改变我们对细胞群的功能表型如何从单个细胞的行为中出现的理解。SCG的一些应用包括发现新的基因网络和新的细胞亚群，转录动力学的精细作图，以及细胞克隆性与其功能表型之间的关系。免疫学是从这些进步中受益最多的领域之一，为我们提供了对哺乳动物免疫的全新见解。在这篇综述中，我们首先介绍了来自单细胞基因组工具，特别是单细胞RNA测序的新免疫学见解。此外，我们还讨论了新的遗传研究设计如何开始解释免疫反应中的个体间差异。最后，我们对能够整合来自同一单细胞的几个读数的新的多组学技术以及这些技术如何将我们对哺乳动物免疫的生物学理解推向一个新的水平进行了展望。
Recent developments in the field of single-cell genomics (SCG) are changing our understanding of how functional phenotypes of cell populations emerge from the behaviour of individual cells. Some of the applications of SCG include the discovery of new gene networks and novel cell subpopulations, fine mapping of transcription kinetics, and the relationships between cell clonality and their functional phenotypes. Immunology is one of the fields that is benefiting the most from such advancements, providing us with completely new insights into mammalian immunity. In this review, we start by covering new immunological insights originating from the use of single-cell genomic tools, specifically single-cell RNA-sequencing. Furthermore, we discuss how new genetic study designs are starting to explain inter-individual variation in the immune response. We conclude with a perspective on new multi-omics technologies capable of integrating several readouts from the same single cell and how such techniques might push our biological understanding of mammalian immunity to a new level.
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自身免疫性疾病基因表达的调控和 CD4+ 效应记忆 T 细胞增殖的遗传基础。
影响因子：
4.5
作者：
Hu X;Kim H;Raj T;Brennan PJ;Trynka G;Teslovich N;Slowikowski K;Chen WM;Onengut S;Baecher-Allan C;De Jager PL;Rich SS;Stranger BE;Brenner MB;Raychaudhuri S
通讯作者：
Raychaudhuri S
影响因子：
30.8
作者：
Fairfax, Benjamin P.;Makino, Seiko;Radhakrishnan, Jayachandran;Plant, Katharine;Leslie, Stephen;Dilthey, Alexander;Ellis, Peter;Langford, Cordelia;Vannberg, Fredrik O.;Knight, Julian C.
通讯作者：
Knight, Julian C.
影响因子：
64.5
作者：
Brodin P;Jojic V;Gao T;Bhattacharya S;Angel CJ;Furman D;Shen-Orr S;Dekker CL;Swan GE;Butte AJ;Maecker HT;Davis MM
通讯作者：
Davis MM
影响因子：
48
作者：
Angermueller C;Clark SJ;Lee HJ;Macaulay IC;Teng MJ;Hu TX;Krueger F;Smallwood S;Ponting CP;Voet T;Kelsey G;Stegle O;Reik W
通讯作者：
Reik W
影响因子：
9.8
作者：
Borel, Christelle;Ferreira, Pedro G.;Antonarakis, Stylianos E.
通讯作者：
Antonarakis, Stylianos E.