Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering.
Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering.
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DOI：
10.1038/s41467-021-27362-w
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发表时间：
2021-12-07
影响因子：
16.6
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Yin J;Lan L;Zhang Y;Ni H;Tan Y;Zhang M;Bai Y;Cheng JX
光热显微镜能够对吸收体（从金属纳米颗粒到化学键）进行高度灵敏的无标记成像。光热信号通常通过使用锁定放大器调制激发光束和解调探测光束来检测。虽然方便，但并未揭示丰富的热动力学。在这里，我们提出了一种无锁定的中红外光热动态成像（PDI）系统，通过 MHz 数字化和调制频率谐波的匹配滤波。在单脉冲激励下以纳秒分辨率获取热动态信息。我们的方法不仅将成像速度提高了两个数量级，而且信噪比比锁定方法提高了四倍，从而实现了单细胞水平的高通量代谢分析。此外，通过利用水和生物分子之间的热衰减差异，在活细胞的中红外PDI中有效分离水背景。这种无损探测化学特异性光热动力学的能力为表征生物和材料样本提供了宝贵的工具。光热显微镜仅限于热动力学成像。在这里，作者介绍了一种无锁定的中红外光热动态成像系统，该系统显着提高了信噪比和成像速度，并演示了单细胞水平的代谢分析和背景去除。
Photothermal microscopy has enabled highly sensitive label-free imaging of absorbers, from metallic nanoparticles to chemical bonds. Photothermal signals are conventionally detected via modulation of excitation beam and demodulation of probe beam using lock-in amplifier. While convenient, the wealth of thermal dynamics is not revealed. Here, we present a lock-in free, mid-infrared photothermal dynamic imaging (PDI) system by MHz digitization and match filtering at harmonics of modulation frequency. Thermal-dynamic information is acquired at nanosecond resolution within single pulse excitation. Our method not only increases the imaging speed by two orders of magnitude but also obtains four-fold enhancement of signal-to-noise ratio over lock-in counterpart, enabling high-throughput metabolism analysis at single-cell level. Moreover, by harnessing the thermal decay difference between water and biomolecules, water background is effectively separated in mid-infrared PDI of living cells. This ability to nondestructively probe chemically specific photothermal dynamics offers a valuable tool to characterize biological and material specimens. Photothermal microscopy is limited for imaging of thermal dynamics. Here, the authors introduce a lock-in free, mid-infrared photothermal dynamic imaging system, which significantly increases SNR and imaging speed, and demonstrate metabolism analysis at single-cell level and background removal.
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DOI：
10.1017/s1551929520000917
发表时间：
2020-05
期刊：
Microscopy today
影响因子：
--
作者：
Kansiz M;Prater C;Dillon E;Lo M;Anderson J;Marcott C;Demissie A;Chen Y;Kunkel G
通讯作者：
Kunkel G
影响因子：
22
作者：
Chatterjee, Rusha;Pavlovetc, Ilia M.;Kuno, Masaru
通讯作者：
Kuno, Masaru
通过拉曼集成中红外光热显微镜对活细胞进行指纹识别
影响因子：
7.4
作者：
Li, Xiaojie;Zhang, Delong;Cheng, Ji-Xin
通讯作者：
Cheng, Ji-Xin
影响因子：
1.9
作者：
Dada, Oluwatosin O.;Feist, Peter E.;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
影响因子：
3.3
作者：
Lad, Mitaben D.;Birembaut, Fabrice;Green, Rebecca J.
通讯作者：
Green, Rebecca J.