Sensory cell proliferation within the olfactory epithelium of developing adult Manduca sexta (Lepidoptera).
Sensory cell proliferation within the olfactory epithelium of developing adult Manduca sexta (Lepidoptera).
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DOI：
10.1371/journal.pone.0000215
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发表时间：
2007-02-14
期刊：
影响因子：
3.7
通讯作者：
Vogt R
中科院分区：
文献类型：
--
作者：
Franco MD;Bohbot J;Fernandez K;Hanna J;Poppy J;Vogt R
昆虫利用一系列表型不同的嗅觉器官（称为嗅觉感受器）来检测多种气味。每个感器包含一个到几个感觉神经元和至少三个支持细胞;这些细胞来自一个或一小群确定的前体细胞的有丝分裂活动。感受器表型由不同的形态和对特定气味的特异性来定义;这些是由相关神经元和支持细胞表达的发育程序的结果，以及由编码诸如气味受体、气味结合蛋白和气味降解酶的蛋白质的嗅觉基因的亚群的选择和表达来定义。本实验研究了成年鼠嗅上皮的发育过程。sexta，鉴定可能建立感器表型的事件。在本研究中，触角组织进行了检查，在第一个三天的18天的发展，一个时期，感觉有丝分裂活动，以前报道发生。每个触角发育为一个圆柱体，向外的感觉上皮分为大约80个重复单位或环。感觉细胞的有丝分裂增殖在化蛹后约20-24小时（a. p.）开始，在衬在每个纤维环的近端和远端边缘的高密度细胞的预先存在的区域中。早在两小时前就观察到这些高密度区，有丝分裂活动开始缓慢，在40-48小时后急剧增加;这种活性被蜕皮甾类增强，但在进入蛹滞育（也是蜕皮甾类敏感的）的动物中不发生。感觉增生开始于沿每个瓣环近端和远端边缘的沿着狭窄区域;这些区域迅速扩张以填充中间瓣环区域。这些区域存在于任何有丝分裂活动之前，作为在化蛹时或之前形成的高密度细胞区域。有丝分裂对蜕皮激素的敏感性可能是协调嗅觉发育与滞育进入的发育选择的一种调节机制。
Insects detect a multitude of odors using a broad array of phenotypically distinct olfactory organs referred to as olfactory sensilla. Each sensillum contains one to several sensory neurons and at least three support cells; these cells arise from mitotic activities from one or a small group of defined precursor cells. Sensilla phenotypes are defined by distinct morphologies, and specificities to specific odors; these are the consequence of developmental programs expressed by associated neurons and support cells, and by selection and expression of subpopulations of olfactory genes encoding such proteins as odor receptors, odorant binding proteins, and odor degrading enzymes. We are investigating development of the olfactory epithelium of adult M. sexta, identifying events which might establish sensilla phenotypes. In the present study, antennal tissue was examined during the first three days of an 18 day development, a period when sensory mitotic activity was previously reported to occur. Each antenna develops as a cylinder with an outward facing sensory epithelium divided into approximately 80 repeat units or annuli. Mitotic proliferation of sensory cells initiated about 20–24 hrs after pupation (a.p.), in pre-existing zones of high density cells lining the proximal and distal borders of each annulus. These high density zones were observed as early as two hr. a.p., and expanded with mitotic activity to fill the mid-annular regions by about 72 hrs a.p. Mitotic activity initiated at a low rate, increasing dramatically after 40–48 hrs a.p.; this activity was enhanced by ecdysteroids, but did not occur in animals entering pupal diapause (which is also ecdysteroid sensitive). Sensory proliferation initiates in narrow zones along the proximal and distal borders of each annulus; these zones rapidly expand to fill the mid-annular regions. These zones exist prior to any mitotic activity as regions of high density cells which form either at or prior to pupation. Mitotic sensitivity to ecdysteroids may be a regulatory mechanism coordinating olfactory development with the developmental choice of diapause entry.
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影响因子：
2
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