A tissue-engineered model of the intestinal lacteal for evaluating lipid transport by lymphatics.
A tissue-engineered model of the intestinal lacteal for evaluating lipid transport by lymphatics.
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DOI：
10.1002/bit.22337
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发表时间：
2009-08-15
影响因子：
3.8
通讯作者：
Swartz, Melody A.
中科院分区：
文献类型：
--
作者：
Dixon, J. Brandon;Raghunathan, Sandeep;Swartz, Melody A.
乳质是所有膳食脂质进入血液循环的入口，但对这些淋巴管对脂质摄取的主动调节知之甚少，并且缺乏体外模型来研究乳质-肠细胞界面。我们描述了一个包含分化的Caco-2细胞和淋巴内皮细胞（LECs）的人肠道微环境的体外模型。我们描述了脂肪酸、脂蛋白、白蛋白和葡聚糖运输的模型，并比较了脂肪酸在体内的定性吸收。我们展示了两种细胞类型和脂肪酸在肠到淋巴方向上的强极化运输的相关形态学特征。我们发现脂质在淋巴内皮单层中的转运率比右旋糖酐或白蛋白高得多，这表明大多数脂质转运是活跃的和细胞内的。荧光脂肪酸Bodipy的共聚焦成像和透射电子显微镜证实了这一点。由于我们的模型概括了体内淋巴细胞-肠细胞界面的关键方面，因此它对于研究淋巴细胞脂质转运的生物学以及作为筛选靶向肠淋巴细胞的药物和纳米颗粒的工具是有用的。
Lacteals are the entry point of all dietary lipids into the circulation, yet little is known about the active regulation of lipid uptake by these lymphatic vessels, and there lacks in vitro models to study the lacteal – enterocyte interface. We describe an in vitro model of the human intestinal microenvironment containing differentiated Caco-2 cells and lymphatic endothelial cells (LECs). We characterize the model for fatty acid, lipoprotein, albumin, and dextran transport, and compare to qualitative uptake of fatty acids into lacteals in vivo. We demonstrate relevant morphological features of both cell types and strongly polarized transport of fatty acid in the intestinal-to-lymphatic direction. We found much higher transport rates of lipid than of dextran or albumin across the lymphatic endothelial monolayer, suggesting most lipid transport is active and intracellular. This was confirmed with confocal imaging of Bodipy, a fluorescent fatty acid, along with transmission electron microscopy. Since our model recapitulates crucial aspects of the in vivo lymphatic-enterocyte interface, it is useful for studying the biology of lipid transport by lymphatics and as a tool for screening drugs and nanoparticles that target intestinal lymphatics.
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影响因子：
15.9
作者：
BLOMSTRAND, R;DAHLBACK, O
通讯作者：
DAHLBACK, O
影响因子：
3.5
作者：
Dixon, JB;Zawieja, DC;Coté, GL
通讯作者：
Coté, GL
影响因子：
3.8
作者：
Dixon, J. Brandon;Gashev, Anatoliy A.;Cote, Gerard L.
通讯作者：
Cote, Gerard L.
影响因子：
4.8
作者：
Luchoomun, J;Hussain, MM
通讯作者：
Hussain, MM
DOI：
10.1073/pnas.0503681102
发表时间：
2005-11-01
影响因子：
11.1
作者：
Helm, CLE;Fleury, ME;Swartz, MA
通讯作者：
Swartz, MA