Conserved features of cohesin binding along fission yeast chromosomes.
Conserved features of cohesin binding along fission yeast chromosomes.
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DOI：
10.1186/gb-2009-10-5-r52
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发表时间：
2009
期刊：
影响因子：
12.3
通讯作者：
Uhlmann F
中科院分区：
文献类型：
--
作者：
Schmidt CK;Brookes N;Uhlmann F
对裂变酵母染色体内聚蛋白定位的高分辨率分析表明，以前被认为是生物体特异性的几个决定因素共同形成了整体分布。内聚蛋白使姐妹染色单体在有丝分裂中精确分离。人们对内聚蛋白如何以及在何处与染色体结合仍然知之甚少，最近的全基因组调查显示，在不同的生物体中，内聚蛋白的染色体关联模式存在明显的差异。在这里，我们提出了高分辨率的分析粘接蛋白定位沿裂变酵母染色体。这揭示了几个决定因素，被认为是不同生物特有的，聚集在一起形成了整体分布。内聚蛋白在染色体装载位点检测到，以内聚蛋白装载物Mis4/Ssl3为特征，在转录活性强的区域。内聚蛋白也通过在装载位点周围的聚合转录终止子的下游易位和积累来响应转录。当细胞进入有丝分裂时，一小部分内聚蛋白在分裂独立的反应中离开染色体，而在后期开始时，大量内聚蛋白在分裂时分离。我们进一步观察到，在有丝分裂过程中，着丝粒内聚蛋白依赖于Aurora B激酶的活性扩散到染色体臂上，强调了内聚蛋白行为的可塑性。我们的研究结果表明，那些被认为是区分生物间黏结蛋白的特征共同定义了裂变酵母黏结蛋白的整体行为。生物之间的明显差异可能反映了对黏合作用的不同方面的强调，而不是不同的原则。
High-resolution analysis of cohesin localization on fission yeast chromosomes reveals that several determinants, previously thought to be organism-specific, come together to shape overall distribution. Cohesin holds sister chromatids together to enable their accurate segregation in mitosis. How, and where, cohesin binds to chromosomes are still poorly understood, and recent genome-wide surveys have revealed an apparent disparity between its chromosomal association patterns in different organisms. Here, we present the high-resolution analysis of cohesin localization along fission yeast chromosomes. This reveals that several determinants, thought specific for different organisms, come together to shape the overall distribution. Cohesin is detected at chromosomal loading sites, characterized by the cohesin loader Mis4/Ssl3, in regions of strong transcriptional activity. Cohesin also responds to transcription by downstream translocation and accumulation at convergent transcriptional terminators surrounding the loading sites. As cells enter mitosis, a fraction of cohesin leaves chromosomes in a cleavage-independent reaction, while a substantial pool of cohesin dissociates when it is cleaved at anaphase onset. We furthermore observe that centromeric cohesin spreads out onto chromosome arms during mitosis, dependent on Aurora B kinase activity, emphasizing the plasticity of cohesin behavior. Our findings suggest that features that were thought to differentiate cohesin between organisms collectively define the overall behavior of fission yeast cohesin. Apparent differences between organisms might reflect an emphasis on different aspects, rather than different principles, of cohesin action.
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DOI：
10.1083/jcb.200605074
发表时间：
2006-08-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ding DQ;Sakurai N;Katou Y;Itoh T;Shirahige K;Haraguchi T;Hiraoka Y
通讯作者：
Hiraoka Y
影响因子：
9.2
作者：
Bernard, Pascal;Drogat, Julie;Javerzat, Jean-Paul
通讯作者：
Javerzat, Jean-Paul
影响因子：
64.8
作者：
Lengronne, A;Katou, Y;Mori, S;Yokobayashi, S;Kelly, GP;Itoh, T;Watanabe, Y;Shirahige, K;Uhlmann, F
通讯作者：
Uhlmann, F
影响因子：
9.8
作者：
Glynn, EF;Megee, PC;Gerton, JL
通讯作者：
Gerton, JL
影响因子：
10.5
作者：
D'Ambrosio, Claudio;Schmidt, Christine Katrin;Uhlmann, Frank
通讯作者：
Uhlmann, Frank