Sensory Conflict Disrupts Activity of the Drosophila Circadian Network.
Sensory Conflict Disrupts Activity of the Drosophila Circadian Network.
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DOI：
10.1016/j.celrep.2016.10.029
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发表时间：
2016-11-08
期刊：
影响因子：
8.8
通讯作者：
Stanewsky R
中科院分区：
文献类型：
--
作者：
Harper REF;Dayan P;Albert JT;Stanewsky R
光和温度的周期性变化使果蝇的生物钟同步，但果蝇的大脑如何整合这两种输入途径来设定昼夜节律时间的问题仍然没有答案。我们通过测试昼夜节律网络对冲突环境输入的弹性来探索多感官线索组合。我们发现，在暴露于感觉冲突期间和之后，失调的光和温度循环会导致野生型苍蝇的日常运动活动发生巨大变化。这种行为的改变与脑时钟神经元周期（PER）振荡幅度的急剧减少以及光敏感和温度敏感神经元亚群之间的不同步有关。行为破坏在很大程度上取决于光和温度信号之间的相位关系。我们的结果代表了果蝇昼夜节律系统中多感觉整合的系统量化，并进一步支持时钟作为耦合振荡亚单位网络的观点。光和温度循环的冲突导致异常的、高原样的运动行为。高原样的行为伴随着分子生物钟的崩溃。在小的光和温度失调时，温度信号占主导地位。在大的光和温度失调时，光信号占主导地位。生物钟允许身体功能在一天的最佳时间发生，需要与当地环境同步。Harper等人研究了生物钟如何对感觉冲突的情况作出反应，并表明在果蝇中，光和温度循环之间的中间（5-7小时）偏移导致活动曲线的改变，这是由分子钟的急剧受损所支撑的。
Periodic changes in light and temperature synchronize the Drosophila circadian clock, but the question of how the fly brain integrates these two input pathways to set circadian time remains unanswered. We explore multisensory cue combination by testing the resilience of the circadian network to conflicting environmental inputs. We show that misaligned light and temperature cycles can lead to dramatic changes in the daily locomotor activities of wild-type flies during and after exposure to sensory conflict. This altered behavior is associated with a drastic reduction in the amplitude of PERIOD (PER) oscillations in brain clock neurons and desynchronization between light- and temperature-sensitive neuronal subgroups. The behavioral disruption depends heavily on the phase relationship between light and temperature signals. Our results represent a systematic quantification of multisensory integration in the Drosophila circadian system and lend further support to the view of the clock as a network of coupled oscillatory subunits. Conflicting light and temperature cycles lead to abnormal, plateau-like locomotor behavior Plateau-like behavior is accompanied by a collapse of the molecular circadian clock Temperature cues dominate during small light and temperature misalignments Light cues dominate during large light and temperature misalignments Circadian clocks allow bodily functions to occur at optimal times of day and need to be synchronized with the local environment. Harper et al. examine how circadian clocks respond to situations of sensory conflict and show that in Drosophila, intermediate (5–7 hr) offsets between light and temperature cycles lead to an altered activity profile, underpinned by a drastically compromised molecular clock.
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