A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions.
A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions.
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DOI：
10.1093/jxb/erq041
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发表时间：
2010-06
影响因子：
6.9
通讯作者：
Kapulnik Y
中科院分区：
文献类型：
--
作者：
Koltai H;LekKala SP;Bhattacharya C;Mayzlish-Gati E;Resnick N;Wininger S;Dor E;Yoneyama K;Yoneyama K;Hershenhorn J;Joel DM;Kapulnik Y
独角孤内酯被认为是一类新的植物激素。它们作为植物生长调节剂和植物相互作用信号分子的作用首先在拟南芥、豌豆和水稻突变体中得到证实，这些突变体在独角麦内酯的产生、释放或感知方面存在缺陷。在番茄（茄）的首个证据独角麦内酯缺乏症是提出在这里。Sl-ORT1，先前被鉴定为对寄生植物orobche具有抗性，具有较低水平的丛枝菌根真菌（Glomus intraradices）定植，可能是由于其诱导菌根菌丝分支的能力降低。对突变根提取物的生化分析表明，它只产生极少量的两种番茄独角酯内酯：茄芦酚和二脱氢角凤尾鱼酚。因此，与野生型（WT）相比，Sl-ORT1中一个被认为参与番茄独角麦内酯合成的关键酶（CCD7）的转录水平降低。Sl-ORT1的枝条表现出增加的侧枝分枝，而外源施用合成的独脚金内酯GR24通过减少侧枝数量恢复了WT表型。嫁接在突变体砧木和接穗之间的WT间砧木的侧枝分枝也明显减少。在这些嫁接植株的根中，CCD7转录水平未被显著诱导，菌根敏感性也未恢复。因此，WT-砧木间嫁接虽然恢复了突变体的芽形态，但并没有恢复突变体的根特性。首个番茄孤孤内酯缺乏突变体的特征支持了孤孤内酯在植物物种多样性中作为抑制侧枝分枝的信使的假设。
Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in tomato (Solanum lycopersicon) of strigolactone deficiency is presented here. Sl-ORT1, previously identified as resistant to the parasitic plant Orobanche, had lower levels of arbuscular mycorrhizal fungus (Glomus intraradices) colonization, possibly as a result of its reduced ability to induce mycorrhizal hyphal branching. Biochemical analysis of mutant root extracts suggested that it produces only minute amounts of two of the tomato strigolactones: solanacol and didehydro-orobanchol. Accordingly, the transcription level of a key enzyme (CCD7) putatively involved in strigolactone synthesis in tomato was reduced in Sl-ORT1 compared with the wild type (WT). Sl-ORT1 shoots exhibited increased lateral shoot branching, whereas exogenous application of the synthetic strigolactone GR24 to the mutant restored the WT phenotype by reducing the number of lateral branches. Reduced lateral shoot branching was also evident in grafted plants which included a WT interstock, which was grafted between the mutant rootstock and the scion. In roots of these grafted plants, the CCD7 transcription level was not significantly induced, nor was mycorrhizal sensitivity restored. Hence, WT-interstock grafting, which restores mutant shoot morphology to WT, does not restore mutant root properties to WT. Characterization of the first tomato strigolactone-deficient mutant supports the putative general role of strigolactones as messengers of suppression of lateral shoot branching in a diversity of plant species.
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影响因子：
4.2
作者：
Akiyama, Kohki;Hayashi, Hideo
通讯作者：
Hayashi, Hideo
影响因子：
9.2
作者：
Booker, J;Auldridge, M;Leyser, O
通讯作者：
Leyser, O
影响因子：
7.4
作者：
Ferguson, Brett J.;Beveridge, Christine A.
通讯作者：
Beveridge, Christine A.
影响因子：
9.8
作者：
Besserer A;Puech-Pagès V;Kiefer P;Gomez-Roldan V;Jauneau A;Roy S;Portais JC;Roux C;Bécard G;Séjalon-Delmas N
通讯作者：
Séjalon-Delmas N
影响因子：
9.4
作者：
GIOVANNETTI, M;MOSSE, B
通讯作者：
MOSSE, B