Correspondence between salivary proteomic pattern and clinical course in primary Sjögren syndrome and non-Hodgkin's lymphoma: a case report.
Correspondence between salivary proteomic pattern and clinical course in primary Sjögren syndrome and non-Hodgkin's lymphoma: a case report.
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DOI：
10.1186/1479-5876-9-188
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发表时间：
2011-11-02
影响因子：
7.4
通讯作者：
Lucacchini A
中科院分区：
文献类型：
--
作者：
Baldini C;Giusti L;Ciregia F;Da Valle Y;Giacomelli C;Donadio E;Ferro F;Galimberti S;Donati V;Bazzichi L;Bombardieri S;Lucacchini A
在过去的几年里，人类蛋白质组学代表了一个有前途的工具，以促进基础和临床科学之间的交流。为了探索唾液蛋白质组学谱与临床反应之间的对应关系，本文中，我们使用蛋白质组学方法分析了患有原发性干燥综合征（pSS）和非霍奇金-MALT型腮腺淋巴瘤的患者在环磷酰胺（CTX）和利妥昔单抗（RTX）标准治疗之前、期间和之后的全唾液。为了鉴定任何区别性治疗性唾液生物标志物，在基线时、第四次输注利妥昔单抗后和缓解时收集患者的全唾液，并结合二维电泳（2DE）和MALDI-TOF/TOF质谱法进行分析。从唾液样品的比较中获得的蛋白质组学结果表明，在唾液中的假定白蛋白、免疫球蛋白J链、IG κ链C区、α-1-抗胰蛋白酶、触珠蛋白和IG α-1链C区的表达中存在几种定性和定量的修饰。这项研究表明，由自身免疫和淋巴增生过程驱动的唾液腺的临床和功能变化可能反映在患者的全唾液蛋白中，从而为唾液蛋白质组学分析在鉴定pSS和非霍奇金淋巴瘤患者的预后和治疗生物标志物方面的潜在用途提供了新的思路。
In the last years human proteomic has represented a promising tool to promote the communication between basic and clinical science. To explore the correspondence between salivary proteomic profile and clinical response, herein, we used a proteomic approach to analyse the whole saliva of a patient with primary Sjögren's Syndrome (pSS) and non-Hodgkin's-MALT type parotid lymphoma before, during and after a standard treatment with cyclophosphamide (CTX) and rituximab (RTX). To identify any discriminatory therapeutic salivary biomarker patient's whole saliva was collected at the baseline, after the fourth infusion of rituximab, and on remission and analysed combining two-dimensional electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry. Proteomic results obtained from the comparison of salivary samples indicated several qualitative and quantitative modifications in the salivary expression of putative albumin, immunoglobulin J chain, Ig kappa chain C region, alpha-1-antitrypsin, haptoglobin and Ig alpha-1 chain C region. This study suggests that clinical and functional changes of the salivary glands driven by autoimmune and lymphoproliferative processes might be reflected in patients' whole saliva proteins, shedding new light on the potential usefulness of salivary proteomic analysis in the identification of prognostic and therapeutic biomarkers for patients with pSS and non Hodgkin's lymphomas.
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影响因子：
--
作者：
Hu, Shen;Zhou, Michael;Jiang, Jiang;Wang, Jianghua;Elashoff, David;Gorr, Sven;Michie, Sara A.;Spijkervet, Fred K. L.;Bootsma, Hendrika;Kallenberg, Cees G. M.;Vissink, Arjan;Horvath, Steve;Wong, David T.
通讯作者：
Wong, David T.
影响因子：
2.3
作者：
Voulgarelis, Michael;Moutsopoulos, Haralampos M.
通讯作者：
Moutsopoulos, Haralampos M.
影响因子：
2.1
作者：
Baturone, R.;Soto, M. J.;Giron-Gonzalez, J. A.
通讯作者：
Giron-Gonzalez, J. A.
影响因子：
--
作者：
Hansen, A;Reiter, K;Dörner, T
通讯作者：
Dörner, T
影响因子：
--
作者：
Peluso, G.;De Santis, M.;Ferraccioli, G. F.
通讯作者：
Ferraccioli, G. F.