A natural human retrovirus efficiently complements vectors based on murine leukemia virus.
A natural human retrovirus efficiently complements vectors based on murine leukemia virus.
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DOI：
10.1371/journal.pone.0003144
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发表时间：
2008-09-04
期刊：
影响因子：
3.7
通讯作者：
Kandel, Eugene S.
中科院分区：
文献类型：
--
作者：
Dong, Beihua;Silverman, Robert H.;Kandel, Eugene S.
鼠白血病病毒（MLV）是一种啮齿动物γ逆转录病毒，作为实验和治疗应用中设计的常见基因递送工具的骨架。最近，在前列腺癌患者中鉴定出一种称为XMRV的传染性γ逆转录病毒。MLV和XMRV基因组之间的相似性表明这两种病毒在同一细胞中存在时可能相互作用。我们测试了XMRV在共感染培养的人细胞后补充复制缺陷型MLV载体的能力。我们观察到，XMRV可以促进这些载体从感染的细胞传播到未感染的细胞。这种功能互补在载体结构中没有任何总体重排的情况下发生，并且共感染的细胞每毫升培养基产生多达104个感染性载体颗粒。需要考虑在体外和体内将基于MLV的载体递送至人细胞时遇到辅助病毒的可能性，以确保此类程序的安全性。
Murine Leukemia Virus (MLV) is a rodent gammaretrovirus that serves as the backbone for common gene delivery tools designed for experimental and therapeutic applications. Recently, an infectious gammaretrovirus designated XMRV has been identified in prostate cancer patients. The similarity between the MLV and XMRV genomes suggests a possibility that the two viruses may interact when present in the same cell. We tested the ability of XMRV to complement replication-deficient MLV vectors upon co-infection of cultured human cells. We observed that XMRV can facilitate the spread of these vectors from infected to uninfected cells. This functional complementation occurred without any gross rearrangements in the vector structure, and the co-infected cells produced as many as 104 infectious vector particles per milliliter of culture medium. The possibility of encountering a helper virus when delivering MLV-based vectors to human cells in vitro and in vivo needs to be considered to ensure the safety of such procedures.
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影响因子：
64.5
作者：
HUANG, CC;HAY, N;BISHOP, JM
通讯作者：
BISHOP, JM
DOI：
10.1007/bf02674280
发表时间：
1997-09-01
期刊：
SOMATIC CELL AND MOLECULAR GENETICS
影响因子：
--
作者：
Kandel, ES;Chang, BD;Roninson, IB
通讯作者：
Roninson, IB
影响因子：
14.9
作者：
Schott, B;Kandel, ES;Roninson, IB
通讯作者：
Roninson, IB
DOI：
10.1073/pnas.92.7.2460
发表时间：
1995-03-28
影响因子：
11.1
作者：
SCHWARTZ, JR;DUESBERG, S;DUESBERG, PH
通讯作者：
DUESBERG, PH
影响因子：
5.3
作者：
DORNBURG, R;TEMIN, HM
通讯作者：
TEMIN, HM