Type II/III cell composition and NCAM expression in taste buds
Type II/III cell composition and NCAM expression in taste buds
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味蕾中 II/III 型细胞组成和 NCAM 表达
DOI：
10.1007/s00441-021-03452-5
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发表时间：
2021
影响因子：
3.6
通讯作者：
Shuitsu Harada
中科院分区：
文献类型：
--
作者：
Eriko Koyanagi-Matsumura;Hirohito Miura;Mitsuru Saito;Shuitsu Harada
味蕾位于舌部的菌状(FF)、叶状(FL)和环状乳突(CV)中，也存在于软腭部(SP)。味蕾中的成熟细长细胞不断从干细胞更新，并分为三种细胞类型，I、II和III。这些细胞类型通常被认为以与味觉区域相对应的特定比例驻留在各自的味蕾中。各种细胞类型标记被用来分析味蕾细胞。NCAM是III型细胞的第一个已建立的标记物，至今仍经常使用。然而，NCAM主要在CV中被检测到，而在其他区域中却没有得到充分的检测。此外，我们先前的数据表明，NCAM可能在II型细胞的未成熟阶段瞬时表达。为了准确评估NCAM作为III型细胞标志物的表达，我们首先分别检测了II型和III型细胞类型标志物IP3R3和CA4，然后使用整体免疫组织化学方法将NCAM与它们进行比较。IP3R3和CA4相互分离，支持这些标记的可靠性。在不同地区的味蕾中，II型和III型细胞的比例差异很大(皮尔逊r=0.442[CV]、0.279[SP]和−=0.011[FF])，这表明II型和III型细胞在各自的味蕾中是相当独立的。NCAM免疫组织化学显示味蕾细胞亚群为NCAM(+)CA4(−)。NCAM(+)CA4(−)细胞在CV中以IP3R3(−)为主，在SP和FF中以IP3R3(+)为主。
Taste buds are localized in fungiform (FF), foliate (FL), and circumvallate (CV) papillae on the tongue, and taste buds also occur on the soft palate (SP). Mature elongate cells within taste buds are constantly renewed from stem cells and classified into three cell types, Types I, II, and III. These cell types are generally assumed to reside in respective taste buds in a particular ratio corresponding to taste regions. A variety of cell-type markers were used to analyze taste bud cells. NCAM is the first established marker for Type III cells and is still often used. However, NCAM was examined mainly in the CV, but not sufficiently in other regions. Furthermore, our previous data suggested that NCAM may be transiently expressed in the immature stage of Type II cells. To precisely assess NCAM expression as a Type III cell marker, we first examined Type II and III cell-type markers, IP3R3 and CA4, respectively, and then compared NCAM with them using whole-mount immunohistochemistry. IP3R3 and CA4 were segregated from each other, supporting the reliability of these markers. The ratio between Type II and III cells varied widely among taste buds in the respective regions (Pearson’sr= 0.442 [CV], 0.279 [SP], and − 0.011 [FF]), indicating that Type II and III cells are contained rather independently in respective taste buds. NCAM immunohistochemistry showed that a subset of taste bud cells were NCAM(+)CA4(−). While NCAM(+)CA4(−) cells were IP3R3(−) in the CV, the majority of them were IP3R3(+) in the SP and FF.
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