The histone H3K27 demethylase REF6/JMJ12 promotes thermomorphogenesis in Arabidopsis.
The histone H3K27 demethylase REF6/JMJ12 promotes thermomorphogenesis in Arabidopsis.
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DOI：
10.1093/nsr/nwab213
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发表时间：
2022-05
影响因子：
20.6
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中科院分区：
文献类型：
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组蛋白H3在Lys27位点（H3K27me3）的动态三甲基化影响基因表达并控制植物发育和环境反应。在拟南芥中，早花6 (REF6)/JUMONJI结构域蛋白12的亲属通过识别特定的DNA基序使H3K27me3去甲基化。然而，对于识别后REF6如何激活靶基因表达，特别是在环境反应中，我们知之甚少。在温暖的环境温度下，植物发生热形态发生，包括加速生长、提前开花和形态变化。本研究表明，在温暖的环境温度下，REF6调节热形态发生，并与转录因子光敏色素相互作用因子4协同激活热反应基因。ref6功能缺失突变体在温暖温度下表现出下胚轴伸长减弱，部分原因是赤霉素20-氧化酶2和BASIC HELIX-LOOP-HELIX 87的下调。REF6酶活性是温暖环境温度反应所必需的。总之，我们的研究结果提供了表观遗传修饰子和转录因子共同作用以响应环境的直接证据。
Dynamic trimethylation of histone H3 at Lys27 (H3K27me3) affects gene expression and controls plant development and environmental responses. In Arabidopsis thaliana, RELATIVE OF EARLY FLOWERING 6 (REF6)/JUMONJI DOMAIN-CONTAINING PROTEIN 12 demethylates H3K27me3 by recognizing a specific DNA motif. However, little is known about how REF6 activates target gene expression after recognition, especially in environmental responses. In response to warm ambient temperature, plants undergo thermomorphogenesis, which involves accelerated growth, early flowering and changes in morphology. Here we show that REF6 regulates thermomorphogenesis and cooperates with the transcription factor PHYTOCHROME INTERACTING FACTOR 4 to synergistically activate thermoresponsive genes under warm ambient temperature. The ref6 loss-of-function mutants exhibited attenuated hypocotyl elongation at warm temperature, partially due to downregulation of GIBBERELLIN 20-OXIDASE 2 and BASIC HELIX-LOOP-HELIX 87. REF6 enzymatic activity is necessary for warm ambient temperature responses. Together, our results provide direct evidence of an epigenetic modifier and a transcription factor working together to respond to the environment.
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