Tumor Necrosis Factor-Alpha in Ileal Mast Cells in Patients with Crohn’s Disease
Tumor Necrosis Factor-Alpha in Ileal Mast Cells in Patients with Crohn’s Disease
复制标题
克罗恩病患者回肠肥大细胞中的肿瘤坏死因子-α
作者：
I. Lilja;C. Gustafson;L. Franzén;R. Sjödahl
背景：有报道称肠道和肠外克罗恩病(CD)经抗肿瘤坏死因子-α(α)抗体治疗后均治愈，这进一步证实了其在CD治疗中的作用。巨噬细胞是肿瘤坏死因子-α的来源之一。肠道肥大细胞也被认为在CD中起作用，但尚不清楚人类回肠肥大细胞是否表达肿瘤坏死因子-α。目的：探讨CD患者和对照组回肠壁肥大细胞是否表达肿瘤坏死因子-α。方法：采用免疫组织化学方法检测CD组(9例组织学正常，6例炎症组)和8例对照组(结肠癌组)全层回肠壁标本中肿瘤坏死因子-α的表达。通过异染色质和抗肥大细胞类胰蛋白酶免疫反应鉴定肥大细胞。结果：在回肠壁的各层中，肥大细胞是肿瘤坏死因子α标记细胞中表达肿瘤坏死因子α免疫反应的主要细胞。肿瘤坏死因子-α标记的肥大细胞在固有肌层的数量明显多于对照组，无论是非炎症性肠炎组(1.7倍，p；0.05)还是炎症性肠炎组(4.6倍，p；0.002)，炎症性肠炎组粘膜下层肥大细胞数均多于非炎症组(1.6倍，p<0.01)，炎症性肠炎组固有层肥大细胞数明显少于非炎症组(0.4倍，p<0.05)。结论：肥大细胞是回肠壁各层肿瘤坏死因子-α的重要来源，回肠黏膜下层和固有肌内肿瘤坏死因子-α阳性的肥大细胞密度增加可能与CD的组织改变和症状有关。
Background: Reports that both intestinal and extraintestinal Crohn’s disease (CD) had healed successfully after treatment with anti-tumor necrosis factor-alpha (TNF-α) antibody have strengthened the hypothesis that it has a role in the treatment of CD. The macrophage is one source of TNF-α. Intestinal mast cells are also thought to have a role in CD, but it is not known if human ileal mast cells express TNF-α. Aim: To find out whether TNF-α is expressed by mast cells in the ileal wall in CD patients and controls. Methods: TNF-α was sought immunohistochemically in full thickness specimens of ileal wall from patients with CD (histologically normal, n = 9; inflamed, n = 6) and controls (patients with colonic cancer, n = 8). Mast cells were identified by metachromasia and anti-mast cell tryptase immunoreactivity. Results: In all layers of the ileal wall, and in every specimen investigated, mast cells were the main cell type that expressed TNF-α immunoreactivity out of the TNF-α-labelled cells. The number of TNF-α- labelled mast cells was greater in the muscularis propria in patients compared with controls, both in uninflamed (1.7-fold, p < 0.05) and in inflamed bowel (4.6-fold, p < 0.002); greater in the submucosa in inflamed compared with uninflamed CD (1.6-fold, p < 0.01), and less in the lamina propria in inflamed compared with uninflamed CD (0.4-fold, p < 0.05). Conclusion: Mast cells are an important source of TNF-α in all layers of the ileal wall, and the increased density of TNF-α-positive mast cells in the submucosa and muscularis propria may contribute to the tissue changes and symptoms in CD.
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克罗恩病 - 使用一微米塑料切片进行肥大细胞定量，用于光学显微镜研究。
影响因子：
--
作者：
Dvorak,AM;Monahan,RA
通讯作者：
Monahan,RA
DOI：
10.1073/pnas.88.10.4220
发表时间：
1991-05-01
影响因子：
11.1
作者：
WALSH, LJ;TRINCHIERI, G;MURPHY, GF
通讯作者：
MURPHY, GF
肿瘤坏死因子和干扰素-γ 诱导狒狒皮肤中不同的内皮激活模式和相关的白细胞积聚。
DOI：
--
发表时间：
1989
期刊：
The American journal of pathology
影响因子：
--
作者：
Munro,JM;Pober,JS;Cotran,RS
通讯作者：
Cotran,RS
影响因子：
4.6
作者：
H. Reinecker;M. Steffen;T. Witthoeft;I. Pflueger;S. Schreiber;R. Macdermott;A. Raedler
通讯作者：
H. Reinecker;M. Steffen;T. Witthoeft;I. Pflueger;S. Schreiber;R. Macdermott;A. Raedler
影响因子：
3.3
作者：
DVORAK, AM;MONAHAN, RA;DICKERSIN, GR
通讯作者：
DICKERSIN, GR