Influence of dose-rate, post-irradiation incubation time and growth factors on interphase cell death by apoptosis and clonogenic survival of human peripheral lymphocytes.
Influence of dose-rate, post-irradiation incubation time and growth factors on interphase cell death by apoptosis and clonogenic survival of human peripheral lymphocytes.
复制标题
剂量率、辐照后孵育时间和生长因子对人外周淋巴细胞凋亡和克隆存活导致的间期细胞死亡的影响。
DOI：
10.1080/095530099139421
复制
发表时间：
1999
影响因子：
2.6
通讯作者：
M. Harms‐Ringdahl
中科院分区：
文献类型：
--
作者：
A. Meijer;A. Saeidi;A. Zelenskaya;S. Czene;F. Granath;M. Harms‐Ringdahl
目的
研究剂量率、照射后孵育时间和生长因子对辐射诱导人外周血淋巴细胞凋亡和生殖细胞死亡的影响。
材料和方法
G 0期淋巴细胞在体外接受高剂量率（HDR，45 Gy/h）或低剂量率（LDR，0.024Gy/h）1-3Gy ~（137）Cs γ射线照射。在第1天（HDR 1）与3戈伊LDR暴露开始同时进行HDR暴露，或在第6天（HDR 6）LDR暴露结束时进行HDR暴露。通过测量（1）细胞膜完整性，（2）形态学变化和（3）细胞大小减少，在照射后不同时间研究细胞凋亡。分析照射后直接接种的细胞（LDR、HDR 6、HDR 1第1天）或照射后孵育5天后接种的细胞（HDR 1第6天）的克隆形成存活率。
结果
与接种第6天的细胞的HDR 1暴露相比，在3戈伊LDR暴露后观察到生殖细胞死亡的显著减少。对于应用的较低剂量，无法在统计学上验证剂量率效应。当将LDR暴露与第6天分析的HDRI进行比较时，观察到所有三种剂量（即1、2和3Gy）的细胞凋亡减少，尽管没有统计学意义。辐射诱导的细胞凋亡被生长因子有效地抵消后24-48小时3戈伊HDR曝光。在G 0期，生长因子对辐射诱导的细胞死亡的预防作用依赖于剂量和照射后时间。当在G 0（HDR 1细胞接种第6天）中长时间照射后孵育后添加生长因子时，与在照射后直接添加生长因子的HDR方案（HDR 1接种第1天和HDR 6）相比，发现生殖细胞死亡显着增加（3戈伊）。
结论
辐射诱导的细胞凋亡的剂量率效应，但没有统计学验证。观察到生殖细胞死亡的显著剂量率效应。然而，当生长因子在HDR照射后直接加入时，这种剂量率效应被逆转。
PURPOSE
To investigate the effects of dose-rate, post-irradiation incubation time and growth factors on radiation-induced interphase cell death by apoptosis and reproductive cell death in human peripheral lymphocytes.
MATERIALS AND METHODS
Lymphocytes in G0-phase were exposed in vitro to 1-3Gy 137Cs gamma-radiation at a high- (HDR, 45Gy/h) or a low dose-rate (LDR, 0.024Gy/h). HDR exposures were performed either on day 1 (HDR1) simultaneously with the start of the 3 Gy LDR exposure, or on day 6 (HDR6) when the LDR exposures ended. Apoptosis was studied at different times after irradiation by measuring (1) cellular membrane integrity, (2) morphological changes and (3) cell size reduction. Clonogenic survival was analysed for cells plated directly after irradiation (LDR, HDR6, HDR1 day 1) or after 5 days post-irradiation incubation (HDR1 day 6).
RESULTS
A significant decrease in reproductive cell death was observed after 3 Gy LDR exposure as compared with the HDR1 exposure for cells plated day 6. For the lower doses applied, the dose-rate effect could not be statistically verified. A decrease in apoptosis for all three doses applied (i.e. 1, 2 and 3Gy) was observed when the LDR exposures were compared with the HDRI analysed day 6, although not of statistical significance. Radiation-induced apoptosis was efficiently counteracted by growth factors up to 24-48 h after 3 Gy HDR exposure. The prevention of radiation-induced cell death by growth factors was dependent on dose and post-irradiation time in G0. When the growth factors were added after a prolonged post-irradiation incubation in G0 (HDR 1 cells plated day 6), a significant increase in reproductive cell death was found (3 Gy) as compared with HDR protocols where the growth factors were added directly after irradiation (HDR 1 plated day 1 and HDR6).
CONCLUSIONS
A dose-rate effect on radiation-induced apoptosis was indicated but not statistically verified. A significant dose-rate effect on reproductive cell death was observed. This dose-rate effect was, however, inverted when growth factors were added directly after the HDR irradiations.
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影响因子：
3.4
作者：
R. E. Langley;S. Quartuccio;P. Kennealey;C. Coleman;E. Bump
通讯作者：
R. E. Langley;S. Quartuccio;P. Kennealey;C. Coleman;E. Bump
淋巴瘤细胞的细胞周期改变、细胞凋亡和对低剂量放射免疫治疗的反应。
DOI：
10.1016/0360-3016(93)90391-8
发表时间：
1993
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Macklis,RM;Beresford,BA;Palayoor,S;Sweeney,S;Humm,JL
通讯作者：
Humm,JL
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DOI：
10.1016/0167-8140(94)90099-x
发表时间：
1994
期刊：
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology
影响因子：
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作者：
Ling,CC;Chen,CH;Li,WX
通讯作者：
Li,WX
低剂量照射对未刺激和 PHA 刺激的人类淋巴细胞亚群的影响。
DOI：
10.1080/09553008814550441
发表时间：
1988
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
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作者：
Stewart,CC;Stevenson,AP;Habbersett,RC
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肿瘤促进剂在体外抑制辐射诱导的细胞凋亡。
DOI：
10.1016/s0006-291x(88)81088-x
发表时间：
1988
影响因子：
3.1
作者：
Tomei,LD;Kanter,P;Wenner,CE
通讯作者：
Wenner,CE