Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance.
Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance.
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DOI：
10.1002/ece3.3425
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发表时间：
2017-11
影响因子：
2.6
通讯作者：
Day KP
中科院分区：
文献类型：
--
作者：
Rougeron V;Tiedje KE;Chen DS;Rask TS;Gamboa D;Maestre A;Musset L;Legrand E;Noya O;Yalcindag E;Renaud F;Prugnolle F;Day KP
人类从非洲迁移出来所产生的强烈的创始人效应导致了疟疾寄生虫恶性疟原虫的单核苷酸多态性（SNP）和微卫星（MS）的地理变异。这在南美洲尤其引人注目，在那里已经确定了两个主要的恶性疟原虫创始种群，据推测它们来自跨大西洋奴隶贸易。鉴于恶性疟原虫血液阶段的主要变异表面抗原作为毒力因子和免疫靶点的重要性，我们决定调查南美洲几个国家中编码“恶性疟原虫红细胞膜蛋白1”（Pf EMP 1）的基因的群体遗传学，以评估该大陆疟疾的传播模式。使用454高通量测序方案完成了来自南美洲5个地点的128株恶性疟原虫分离株var基因DBLα结构域的深度测序。变异DBLα序列的显著地理变异与SNPs和MS标记相似。哥伦比亚和法属圭亚那具有不同的变异DBLα序列，而秘鲁和委内瑞拉则表现出混合。这种地理变异的重要性，群体免疫和疟疾疫苗接种进行了讨论。
Strong founder effects resulting from human migration out of Africa have led to geographic variation in single nucleotide polymorphisms (SNPs) and microsatellites (MS) of the malaria parasite, Plasmodium falciparum. This is particularly striking in South America where two major founder populations of P. falciparum have been identified that are presumed to have arisen from the transatlantic slave trade. Given the importance of the major variant surface antigen of the blood stages of P. falciparum as both a virulence factor and target of immunity, we decided to investigate the population genetics of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (Pf EMP1) among several countries in South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using a 454 high throughput sequencing protocol. Striking geographic variation in var DBLα sequences, similar to that seen for SNPs and MS markers, was observed. Colombia and French Guiana had distinct var DBLα sequences, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.
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DOI：
10.1093/bioinformatics/btq365
发表时间：
2010-09-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Balzer S;Malde K;Lanzén A;Sharma A;Jonassen I
通讯作者：
Jonassen I
Biopython：用于计算分子生物学和生物信息学的免费 Python 工具。
DOI：
10.1093/bioinformatics/btp163
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Cock PJ;Antao T;Chang JT;Chapman BA;Cox CJ;Dalke A;Friedberg I;Hamelryck T;Kauff F;Wilczynski B;de Hoon MJ
通讯作者：
de Hoon MJ
影响因子：
64.5
作者：
BARUCH, DI;PASLOSKE, BL;HOWARD, RJ
通讯作者：
HOWARD, RJ
影响因子：
8.8
作者：
Chao, A;Chazdon, RL;Shen, TJ
通讯作者：
Shen, TJ
影响因子：
6.7
作者：
Barry AE;Leliwa-Sytek A;Tavul L;Imrie H;Migot-Nabias F;Brown SM;McVean GA;Day KP
通讯作者：
Day KP