Interaction of micron and nano-sized particles with cells of the dura mater.
Interaction of micron and nano-sized particles with cells of the dura mater.
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DOI：
10.1002/jbm.b.33129
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发表时间：
2014-10
影响因子：
3.4
通讯作者：
Ingham, Eileen
中科院分区：
文献类型：
--
作者：
Papageorgiou, Iraklis;Marsh, Rainy;Tipper, Joanne L.;Hall, Richard M.;Fisher, John;Ingham, Eileen
椎间全椎间盘置换术（TDR）用于治疗退行性椎间盘疾病。然而，人们担心它们可能会因磨损而长期失效。TDR磨损的不良影响可能会在硬脑膜和周围组织中表现出来。本研究的目的是研究硬脑膜的生理结构，分离驻留的硬脑膜上皮细胞和基质细胞，并分析这些细胞内化模型聚合物颗粒的能力。猪硬脑膜是一种富含胶原的结构，在最外层的上皮细胞层内包含规则排列的成纤维细胞。分离的硬脑膜上皮细胞具有内皮细胞特征（血管性血友病因子、CD 31、E-钙粘蛋白和桥粒斑蛋白阳性）和屏障功能，而成纤维细胞的胶原蛋白I和III、腱生蛋白和肌动蛋白阳性。硬脑膜细胞吸收模型颗粒的能力取决于颗粒的大小。纳米尺寸的颗粒很容易穿透这两种类型的细胞。然而，硬脑膜成纤维细胞吞噬微米级颗粒的速度比硬脑膜上皮细胞高得多。该研究表明，硬脑膜上皮细胞可能对微米级颗粒的渗透提供一些屏障，但对纳米级颗粒则没有。© 2014作者。生物医学材料研究杂志B部分：应用生物材料，由Wiley期刊公司出版。J Biomed Mater Res Part B：Appl Biomater，102 B：1496-1505，2014.
Intervertebral total disc replacements (TDR) are used in the treatment of degenerative spinal disc disease. There are, however, concerns that they may be subject to long-term failure due to wear. The adverse effects of TDR wear have the potential to manifest in the dura mater and surrounding tissues. The aim of this study was to investigate the physiological structure of the dura mater, isolate the resident dural epithelial and stromal cells and analyse the capacity of these cells to internalise model polymer particles. The porcine dura mater was a collagen-rich structure encompassing regularly arranged fibroblastic cells within an outermost epithelial cell layer. The isolated dural epithelial cells had endothelial cell characteristics (positive for von Willebrand factor, CD31, E-cadherin and desmoplakin) and barrier functionality whereas the fibroblastic cells were positive for collagen I and III, tenascin and actin. The capacity of the dural cells to take up model particles was dependent on particle size. Nanometer sized particles readily penetrated both types of cells. However, dural fibroblasts engulfed micron-sized particles at a much higher rate than dural epithelial cells. The study suggested that dural epithelial cells may offer some barrier to the penetration of micron-sized particles but not nanometer sized particles. © 2014 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1496–1505, 2014.
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影响因子：
7.5
作者：
Harris ES;Nelson WJ
通讯作者：
Nelson WJ
DOI：
10.1083/jcb.201009141
发表时间：
2011-03-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baum B;Georgiou M
通讯作者：
Georgiou M
DOI：
10.1097/01.bsd.0000172361.07479.6b
发表时间：
2005-08-01
影响因子：
--
作者：
Lemaire, JP;Carrier, H;Lavaste, F
通讯作者：
Lavaste, F
影响因子：
3.9
作者：
Boisen, Louise;Drasbek, Kim Ryun;Kristensen, Peter
通讯作者：
Kristensen, Peter
DOI：
10.1007/s10856-006-0015-z
发表时间：
2007-05-01
影响因子：
3.7
作者：
Brown, Christopher;Williams, Sophie;Ingham, Eileen
通讯作者：
Ingham, Eileen