Erythrocyte glutathione transferase in kidney transplantation: a probe for kidney detoxification efficiency.
Erythrocyte glutathione transferase in kidney transplantation: a probe for kidney detoxification efficiency.
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DOI：
10.1038/s41419-018-0289-3
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发表时间：
2018-02-19
影响因子：
9
通讯作者：
Ricci G
中科院分区：
文献类型：
--
作者：
Bocedi A;Noce A;Rovella V;Marrone G;Cattani G;Iappelli M;De Paolis P;Iaria G;Sforza D;Gallù M;Tisone G;Di Daniele N;Ricci G
在血液毒性增加的情况下，红细胞谷胱甘肽转移酶（e-GST）会过度表达，其水平与肾脏疾病的进展相关。因此，它代表了肾脏对抗循环毒素效率的探针。我们测量了来自活体和尸体捐赠者的肾移植患者的 e-GST 活性，将其水平与肾功能的生化参数相关联，并使用一种新的简单程序测量氧化白蛋白的水平作为氧化应激的探针。有趣的是，与健康受试者 (N = 80) ( 5.6 U/gHb) 相比，尸体捐赠者移植患者 (N = 153) 的 e-GST 活性非常高 (11.7 U/gHb)。在接受活体捐赠者肾脏移植的患者中观察到较低的值 (N = 16) (9.8 U/gHb)。除类固醇外，尚未发现与免疫抑制治疗和常规临床和实验室参数的相关性。此外，与活体捐赠者 (36%) 相比，尸体捐赠者 (53%) 的移植患者的血清氧化白蛋白 (揭示氧化应激) 显着较高。总体而言，这些数据表明，与来自活体捐赠者的肾脏相比，大多数来自尸体的移植肾脏失去了部分针对循环毒素的解毒能力，并遭受了相关的氧化应激。一份病例报告表明，e-GST 可能代表早期移植物排斥反应的一个非常早期的标志。总之，e-GST可用于检查移植后过程中肾脏解毒能力的下降或维持。
Erythrocyte glutathione transferase (e-GST) is overexpressed in case of increased blood toxicity and its level correlates with the kidney disease progression. Thus, it represents a probe of kidney efficiency against circulating toxins. We measured the activity of e-GST in patients with transplant kidney from living and cadaver donors, correlated its level to biochemical parameters of kidney function, and measured the level of oxidized albumin as a probe of oxidative stress using a new simple procedure. Interestingly, the activity of e-GST in transplant patients from cadaver donors (N = 153) is very high (11.7 U/gHb) compared to healthy subjects (N = 80) ( 5.6 U/gHb). Lower values were observed in transplant patients with kidney from living donors (N = 16) (9.8 U/gHb). Except for steroids, no correlation has been found with the immunosuppressive therapies and routine clinical and laboratory parameters. Also serum oxidized albumin, which reveals oxidative stress, is significantly higher in transplant patients from cadaver donors (53%) compared to that from living donors (36%). Overall, these data indicate that most of transplant kidneys from cadavers lost part of the detoxifying power against circulating toxins and suffer a relevant oxidative stress compared to those coming from living donors. A case report suggests that e-GST could represent a very early marker of incipient graft rejection. In conclusion, e-GST may be used to check the decline or maintenance of the kidney detoxification competence during post-transplantation course.
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影响因子：
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作者：
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