Three Dimensional Printing Bilayer Membrane Scaffold Promotes Wound Healing
Three Dimensional Printing Bilayer Membrane Scaffold Promotes Wound Healing
复制标题
三维打印双层膜支架促进伤口愈合
DOI：
10.3389/fbioe.2019.00348
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发表时间：
2019-11
影响因子：
5.7
通讯作者：
Binbin Sun
中科院分区：
文献类型：
--
作者：
Shoubao Wang;Yao Xiong;Jingting Chen;Abdulsamad Ghanem;Yinmin Wang;Jun Yang;Binbin Sun
全层皮肤伤口是常见的，可能是一个沉重的身体和经济负担。随着三维（3D）打印技术的发展，已经制造出用于皮肤伤口愈合和再生的皮肤样结构。虽然在血管网络可以很好地构建之前，3D打印皮肤具有巨大的潜力和巨大的优势，但不建议将活细胞用于3D皮肤打印。在此，我们设计并打印了一个双层膜（BLM）。支架组成的外部聚（乳酸-共-乙醇酸）（PLGA）膜和。一个较低的藻酸盐水凝胶层，分别模仿皮肤表皮和。真皮。BLM支架的多孔藻酸盐水凝胶促进了体外细胞粘附和增殖，而PLGA膜阻止了细菌入侵并保持了水凝胶的含水量。将该双层支架与PLGA、藻酸盐水凝胶和未处理的缺损进行了体内皮肤再生的比较。采用组织病理学和免疫组织化学方法对组织样本进行分析。此外，还测量了胶原标志物[1型胶原α 1（COL 1a 1）和3型胶原α 1（COL 3a 1）]和炎症标志物[白细胞介素-1b（IL-1b）以及肿瘤坏死因子（TNF-α）]的mRNA表达水平。总之，BLM支架的应用通过增加新血管形成和促进胶原蛋白I/III沉积而导致最高水平的最佳皮肤再生。综上所述，3D打印的BLMscaffolds可以促进伤口愈合，并且非常适合作为伤口敷料或皮肤替代品的广泛应用。
Full-thickness skin wounds are common and could be a heavy physical and economic.burden. With the development of three dimensional (3D) printing technology, skin-like.constructs have been fabricated for skin wound healing and regeneration. Although.the 3D printed skin has great potential and enormous advantages before vascular.networks can be well-constructed, living cells are not recommended for 3D skin printing.for in vivo applications. Herein, we designed and printed a bilayer membrane (BLM).scaffold consisting of an outer poly (lactic-co-glycolic acid) (PLGA) membrane and.a lower alginate hydrogel layer, which respectively mimicked the skin epidermis and.dermis. The multi-porous alginate hydrogel of the BLM scaffolds promoted cell adhesion.and proliferation in vitro, while the PLGA membrane prevented bacterial invasion and.maintained the moisture content of the hydrogel. Skin regeneration using the bilayer.scaffold was compared with that of PLGA, alginate hydrogel and the untreated defect in.vivo. Tissue samples were analyzed using histopathological and immunohistochemical.staining of CD31. In addition, mRNA expression levels of collagen markers [collagen.type 1 alpha 1 (COL1a1) and collagen type 3 alpha 1 (COL3a1)] and inflammatory.markers [interleukin-1b (IL-1b), as well as tumor necrosis factor (TNF-a)] were measured..Conclusively, the application of BLM scaffold resulted in highest levels of best skin.regeneration by increasing neovascularization and boosting collagen I/III deposition..Taken together, the 3D-printed BLMscaffolds can promote wound healing, and are highly.suitable for a wide range of applications as wound dressings or skin substitutes.
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影响因子：
6
作者：
Skardal A;Mack D;Kapetanovic E;Atala A;Jackson JD;Yoo J;Soker S
通讯作者：
Soker S
影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
9.7
作者：
Zhao X;Sun X;Yildirimer L;Lang Q;Lin ZYW;Zheng R;Zhang Y;Cui W;Annabi N;Khademhosseini A
通讯作者：
Khademhosseini A
DOI：
10.1016/j.biopha.2018.06.176
发表时间：
2018-12
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
Yajuan Song;Zhou Yu;Baoqiang Song;Shuzhong Guo;L. Lei;Xianjie Ma;Yingjun Su
通讯作者：
Yajuan Song;Zhou Yu;Baoqiang Song;Shuzhong Guo;L. Lei;Xianjie Ma;Yingjun Su
影响因子：
10.5
作者：
J. Schulz;R. Tompkins;J. Burke
通讯作者：
J. Schulz;R. Tompkins;J. Burke