Effects of age on H1N1-specific serum IgG1 and IgG3 levels evaluated during the 2011-2012 influenza vaccine season.
Effects of age on H1N1-specific serum IgG1 and IgG3 levels evaluated during the 2011-2012 influenza vaccine season.
复制标题
DOI：
10.1186/1742-4933-10-14
复制
发表时间：
2013-04-22
期刊：
影响因子：
--
通讯作者：
Blomberg BB
中科院分区：
文献类型：
--
作者：
Frasca D;Diaz A;Romero M;Mendez NV;Landin AM;Blomberg BB
我们以前曾报道过，通过血凝抑制试验和ELISA测定，血清抗体对2009年H1N1大流行的反应与年龄相关。本研究扩展了这些观察结果，并评估了2011-2012年接种疫苗的不同年龄健康个体中IgG亚类的分布。2011-2012年接种季节的特点是连续第三年接种了含有2009年H1N1大流行毒株的疫苗。我们所有的受试者之前都接种过疫苗，因此在10岁时得到了免疫保护。然而，年龄的增长削弱了对H1N1的血清抗体反应，因为年轻人接种疫苗后抗体滴度增加，而老年人接种疫苗后抗体滴度减少。反应的高峰出现在第7天（t7），与通常在第21-28天看到的相反，表明记忆反应的特征是诱导半衰期较短的IgG亚类。我们假设，半衰期短得多的IgG3反应可能更有代表性。在两个年龄组中，抗体主要是IgG1亚类，尽管也诱导了强大的IgG3反应，并占总体反应的很大比例。IgG2和IgG4抗体水平不明显。我们发现IgG3的比例（40-50%）比之前的文献（不到10%）高得多。为了解释这是否与特定的细胞因子谱相关，我们在体外测量了h1n1诱导的T细胞因子，发现IgG3水平与TNF-α和IL-6呈正相关。此外，激活诱导的胞苷脱氨酶（AID） mRNA表达，作为体内最佳疫苗反应的预测性生物标志物，被发现与IgG3和IgG1显著相关，这与我们之前在总IgG中所显示的相似。在2011-2012年流感季，2009年H1N1大流行毒株连续第三年出现在疫苗中，因此每个人在10岁时都得到了血清保护。反应的峰值出现在t7，表明记忆反应的特征是IgG3的强烈诱导，而IgG3与TNF-α和IL-6的产生有关。IgG1和IgG3的反应随年龄的增长而降低。AID被证实是最佳疫苗反应的预测性生物标志物。
We have previously reported an age-related impairment in the serum antibody response to pandemic (p)2009 H1N1, measured by hemagglutination inhibition assay and ELISA. The present study extends these observations and evaluates IgG subclass distribution in healthy individuals of different ages vaccinated during the 2011–2012 season. The 2011-2012 vaccination season was characterized by a vaccine containing the pandemic (p)2009 H1N1 strain for the third consecutive year. All of our subjects were previously immunized, and therefore seroprotected at t0. Nevertheless, aging impaired the serum antibody response to H1N1, as antibody titers increased after vaccination in young and less in elderly individuals. The peak of the response was at day 7 (t7), in contrast with what is usually seen at day 21–28, suggesting a memory response characterized by the induction of an IgG subclass with a shorter half-life. We hypothesized that the IgG3 response, with its much shorter half-life, might be more represented. Antibodies were predominantly of the IgG1 subclass in both age groups, although a robust IgG3 response was also induced and accounted for a significant proportion of the overall response. IgG2 and IgG4 antibodies were at indiscernible levels. We showed a much higher percentage of IgG3 (40–50%) than previously in the literature (less than 10%). To explain if this was associated with a particular cytokine profile, we measured H1N1-induced T cell cytokines in vitro and found that IgG3 levels were positively correlated with TNF-α and IL-6. Moreover, activation-induced cytidine deaminase (AID) mRNA expression, a predictive biomarker of optimal in vivo vaccine response, was found to significantly correlate with IgG3 and also with IgG1 similar to what we have shown previously for total IgG. In the 2011–2012 season, the pandemic (p)2009 H1N1 strain was present in the vaccine for the third consecutive year and therefore each individual was seroprotected at t0. The peak of the response was at t7, suggesting a memory response characterized by a robust induction of IgG3, which was associated with TNF-α and IL-6 production. Both IgG1 and IgG3 responses were decreased by age. AID was confirmed to be a predictive biomarker of optimal vaccine responses.
登录
查看更多内容
影响因子：
13.1
作者：
McElhaney, Janet E.
通讯作者：
McElhaney, Janet E.
影响因子：
6.7
作者：
Khurana, Surender;Frasca, Daniela;Golding, Hana
通讯作者：
Golding, Hana
影响因子：
4.4
作者：
Frasca, Daniela;Diaz, Alain;Blomberg, Bonnie B.
通讯作者：
Blomberg, Bonnie B.
DOI：
10.1001/jama.277.9.728
发表时间：
1997-01-01
期刊：
JAMA (Journal of the American Medical Association)
影响因子：
--
作者：
Ferrucci, Luigi;Guralnik, Jack M.;Havlik, Richard J.
通讯作者：
Havlik, Richard J.
影响因子：
3.7
作者：
Ito, T;Suzuki, Y;Kawaoka, Y
通讯作者：
Kawaoka, Y