Acinetobacter, Aeromonas and Trichococcus populations dominate the microbial community within urban sewer infrastructure.
Acinetobacter, Aeromonas and Trichococcus populations dominate the microbial community within urban sewer infrastructure.
复制标题
DOI：
10.1111/j.1462-2920.2012.02757.x
复制
发表时间：
2012-09
影响因子：
5.1
通讯作者：
McLellan SL
中科院分区：
文献类型：
--
作者：
Vandewalle JL;Goetz GW;Huse SM;Morrison HG;Sogin ML;Hoffmann RG;Yan K;McLellan SL
我们评估的人口结构和时间动态的优势社区成员内的污水进水从两个污水处理厂（WWTPs）在密尔沃基，WI。我们从38个流入样品和两个在卫生下水道系统上游采集的样品的16 S rRNA基因的V6高变区产生了>1.1M的细菌pyrotag序列。只有一小部分来自流入物样品的pyrotags（~15%）匹配来自人粪便样品的序列。污水样品的粪便成分包括丰富的pyrotag人口从乳球菌和肠杆菌科相对于他们的分数表示在人类粪便样品。与代表粪便细菌如毛螺菌科和拟杆菌的大量不同的pyrotags相反，只有一个或两个独特的V6序列代表不动杆菌属、毛球菌属和气单胞菌属，它们共同占总污水群落的近35%。两个占主导地位的不动杆菌V6 pyrotags（指定Acineto标签1和Acineto标签2）在3年的时间内与季节性模式呈反比波动，这表明两个不同的不动杆菌种群对系统中的生态强迫有不同的反应。V6 pyrotags中的单核苷酸变化解释了这些群体中的差异，并对应于基于全长序列的两个遗传学上不同的进化枝。小波函数，来自时间波动的数学模型的分析表明，其他丰富的下水道相关的人口，包括毛球菌和气单胞菌的时间模式相似的Acineto标签1或Acineto标签2。人口与相关的时间波动被发现显着相关的相同的污水处理厂变量（5天的生化需氧量，流量，氨，总磷，悬浮固体）。这些发现表明，V6序列的微小差异可以代表遗传学和生态学上不同的类群。这项工作提供了深入了解城市下水道基础设施的定义环境内的微生物群落组成和动态。
We evaluated the population structure and temporal dynamics of the dominant community members within sewage influent from two wastewater treatment plants (WWTPs) in Milwaukee, WI. We generated >1.1M bacterial pyrotag sequences from the V6 hypervariable region of 16S rRNA genes from 38 influent samples and two samples taken upstream in the sanitary sewer system. Only a small fraction of pyrotags from influent samples (~15%) matched sequences from human fecal samples. The fecal components of the sewage samples included enriched pyrotag populations from Lactococcus and Enterobacteriaceae relative to their fractional representation in human fecal samples. In contrast to the large number of distinct pyrotags that represent fecal bacteria such as Lachnospiraceae and Bacteroides, only one or two unique V6 sequences represented Acinetobacter, Trichococcus and Aeromonas, which collectively account for nearly 35% of the total sewage community. Two dominant Acinetobacter V6 pyrotags (designated Acineto tag 1 and Acineto tag 2) fluctuated inversely with a seasonal pattern over a 3-year period, suggesting two distinct Acinetobacter populations respond differently to ecological forcings in the system. A single nucleotide change in the V6 pyrotags accounted for the difference in these populations and corresponded to two phylogenically distinct clades based on full-length sequences. Analysis of wavelet functions, derived from a mathematical model of temporal fluctuations, demonstrated that other abundant sewer associated populations including Trichococcus and Aeromonas had temporal patterns similar to either Acineto tag 1 or Acineto tag 2. Populations with related temporal fluctuations were found to significantly correlate with the same WWTP variables (5-day BOD, flow, ammonia, total phosphorous, and suspended solids). These findings illustrate that small differences in V6 sequences can represent phylogenetically and ecologically distinct taxa. This work provides insight into microbial community composition and dynamics within the defined environment of urban sewer infrastructure.
登录
查看更多内容
影响因子：
12.8
作者：
Ferguson, CM;Coote, BG;Stevenson, IM
通讯作者：
Stevenson, IM
影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
DOI：
10.1186/1746-1448-5-2
发表时间：
2009-02-18
期刊：
Saline systems
影响因子：
--
作者：
Ben-Dov, Eitan;Kushmaro, Ariel;Brenner, Asher
通讯作者：
Brenner, Asher