Interaction of oral bacteria with gingival epithelial cell multilayers.
Interaction of oral bacteria with gingival epithelial cell multilayers.
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DOI：
10.1111/j.2041-1014.2011.00609.x
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发表时间：
2011-06
影响因子：
3.7
通讯作者：
Lamont RJ
中科院分区：
文献类型：
--
作者：
Dickinson BC;Moffatt CE;Hagerty D;Whitmore SE;Brown TA;Graves DT;Lamont RJ
原代牙龈上皮细胞多层培养，作为研究与健康和牙周病相关的口腔细菌相互作用的模型。在低钙培养基中，维持在气液界面的多层细胞显示出连接上皮的分化和细胞角蛋白特性。用荧光标记的牙龈卟啉单胞菌、放线菌聚集菌、核梭菌或戈登链球菌感染多层膜，通过共聚焦显微镜和定量图像分析确定细菌的相关性。牙龈假单胞菌侵入细胞内并在细胞间扩散。放线菌属和具核菌属仍在胞外，并通过多层膜表现出胞间运动。高氏葡萄球菌保持在细胞外，主要与细胞表层相关。通过上皮电阻测量，没有一种细菌破坏屏障功能。牙龈假单胞菌不引起促炎细胞因子的分泌。放线菌A.放线菌comitans和S. gordonii诱导IL-1β、TNF-α、IL-6和IL-8分泌；和F. nucleatum刺激IL-1β和TNF-α的产生。感染24 h后，放线菌、具核单胞菌和戈尔多氏单胞菌的细胞凋亡水平升高，而牙龈单胞菌的细胞凋亡水平不升高。结果表明，具有致病潜力的微生物能够穿过上皮，而共生菌则不能。此外，不同的宿主反应表征了接合上皮和口腔细菌之间的相互作用。
Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air-liquid interface in low calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. P. gingivalis invaded intracellularly and spread cell to cell. A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement through the multilayer. S. gordonii remained extracellular and predominantly associated with the superficial cell layer. None of the bacterial species disrupted barrier function as measured by transepithelial electrical resistance. P. gingivalis did not elicit secretion of proinflammatory cytokines. However, A. actinomycetemcomitans and S. gordonii induced IL-1β, TNF-α, IL-6 and IL-8 secretion; and F. nucleatum stimulated production of IL-1β and TNF-α. A. actinomycetemcomitans, F. nucleatum and S. gordonii, but not P. gingivalis, increased levels of apoptosis after 24 h infection. The results indicate that the organisms with pathogenic potential were able to traverse the epithelium, while the commensal bacteria did not. In addition, distinct host responses characterized the interaction between the junctional epithelium and oral bacteria.
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影响因子：
4.3
作者：
Huang, GTJ;Haake, SK;Park, NH
通讯作者：
Park, NH
影响因子：
3.4
作者：
Handfield, M;Mans, JJ;Lamont, RJ
通讯作者：
Lamont, RJ
影响因子：
15.9
作者：
Kagnoff, MF;Eckmann, L
通讯作者：
Eckmann, L
影响因子：
3.1
作者：
Han, YW;Shi, WY;Genco, RJ
通讯作者：
Genco, RJ
影响因子：
3.1
作者：
Hasegawa, Yoshiaki;Mans, Jeffrey J.;Lamont, Richard J.
通讯作者：
Lamont, Richard J.