Study of charge-dependent transport and toxicity of peptide-functionalized silver nanoparticles using zebrafish embryos and single nanoparticle plasmonic spectroscopy.
Study of charge-dependent transport and toxicity of peptide-functionalized silver nanoparticles using zebrafish embryos and single nanoparticle plasmonic spectroscopy.
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使用斑马鱼胚胎和单纳米颗粒等离子体光谱研究肽功能化银纳米颗粒的电荷依赖性运输和毒性。
DOI：
10.1021/tx400087d
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发表时间：
2013-06-17
影响因子：
4.1
通讯作者：
Xu XH
中科院分区：
文献类型：
--
作者：
Lee KJ;Browning LM;Nallathamby PD;Xu XH
纳米材料具有异常高的表面积与体积比，以及表面决定的物理化学性质。为了合理设计生物相容性纳米材料以用于各种应用，了解其表面依赖性毒性至关重要。在这项研究中，我们用三种生物相容性肽（CALNNK，CALNNS，CALNNE）功能化银纳米颗粒（Ag NPs，直径11.7 ± 2.7 nm）的表面，分别制备带正电荷（Ag-CALNNK NPs+ β），负电荷（Ag-CALNNS NPs−2 β）和带更多负电荷的NPs（Ag-CALNNE NPs−4 β）。每种肽在其C末端的单个氨基酸上不同，这最小化了肽序列的影响，并用作在pH 4-10下在NP表面上产生正电荷、中性电荷和负电荷的模型分子。我们已经研究了他们的电荷依赖性运输到早期发育（卵裂阶段）斑马鱼胚胎和胚胎发育的影响，使用暗场光学显微镜和光谱（DFOMS）。我们发现，所有三个Ag-肽纳米粒子被动扩散到胚胎通过其绒毛膜孔运河，并留在胚胎内，在整个发展（120小时），显示电荷独立的扩散模式和电荷依赖的扩散系数。值得注意的是，NP对胚胎发育产生电荷依赖性毒性作用，表明Ag-CALNNK NP + β（带正电荷）是最生物相容的，而Ag-CALNNE NP-4 β（带更多负电荷）是最毒性的。通过与我们先前对相同大小的柠檬酸化Ag和Au NPs的研究进行比较，Ag-肽NPs比柠檬酸化Ag NPs具有更好的生物相容性，并且几乎与Au NPs一样具有生物相容性，显示纳米毒性依赖于NPs的表面电荷、表面官能团和化学组成。该研究还展示了单NP等离子体光谱用于定量分析体内和组织中的单NP的强大应用，并揭示了合理设计生物相容性NP的可能性。
Nanomaterials possess unusually high surface area-to-volume ratios, and surface-determined physicochemical properties. It is essential to understand their surface-dependent toxicity in order to rationally design biocompatible nanomaterials for a wide variety of applications. In this study, we have functionalized the surfaces of silver nanoparticles (Ag NPs, 11.7 ± 2.7 nm in diameters) with three biocompatible peptides (CALNNK, CALNNS, CALNNE) to prepare positively (Ag-CALNNK NPs+ζ), negatively (Ag-CALNNS NPs−2ζ), and more negatively charged NPs (Ag-CALNNE NPs−4ζ), respectively. Each peptide differs in a single amino acid at its C-terminus, which minimizes the effects of peptide sequences and serves as a model molecule to create positive, neutral and negative charges on the surface of the NPs at pH 4–10. We have studied their charge-dependent transport into early-developing (cleavage-stage) zebrafish embryos and their effects on embryonic development using dark-field optical microscopy and spectroscopy (DFOMS). We found that all three Ag-peptide NPs passively diffused into the embryos via their chorionic pore canals, and stayed inside the embryos throughout their entire development (120 h), showing charge-independent diffusion modes and charge-dependent diffusion coefficients. Notably, the NPs create charge-dependent toxic effects on embryonic development, showing that the Ag-CALNNK NPs+ζ (positively charged) are the most biocompatible while the Ag-CALNNE NPs–4ζ (more negatively charged) are the most toxic. By comparing with our previous studies of the same sized citrated Ag and Au NPs, the Ag-peptide NPs are much more biocompatible than the citrated Ag NPs, and nearly as biocompatible as the Au NPs, showing the dependence of nanotoxicity upon the surface charges, surface functional groups and chemical compositions of the NPs. This study also demonstrates powerful applications of single NP plasmonic spectroscopy for quantitative analysis of single NPs in vivo and in tissues, and reveals the possibility of rational design of biocompatible NPs.
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影响因子：
8.6
作者：
Doty, RC;Tshikhudo, TR;Fernig, DG
通讯作者：
Fernig, DG
影响因子：
6.7
作者：
Browning LM;Lee KJ;Huang T;Nallathamby PD;Lowman JE;Xu XH
通讯作者：
Xu XH
影响因子：
11.4
作者：
El Badawy, Amro M.;Silva, Rendahandi G.;Tolaymat, Thabet M.
通讯作者：
Tolaymat, Thabet M.
影响因子：
17.1
作者：
AshaRani, P. V.;Mun, Grace Low Kah;Valiyaveettil, Suresh
通讯作者：
Valiyaveettil, Suresh
影响因子：
6.7
作者：
Huang, Tao;Browning, Lauren M.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy