Development of a Highly Selective and Sensitive Fluorescent Probe for Imaging RNA Dynamics in Live Cells.
Development of a Highly Selective and Sensitive Fluorescent Probe for Imaging RNA Dynamics in Live Cells.
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一种高选择性、高灵敏度的活细胞RNA动态成像荧光探针的研制。
DOI：
10.3390/molecules27206927
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发表时间：
2022-10-15
期刊：
影响因子：
4.6
通讯作者：
Chen, Xiu-Cai
中科院分区：
文献类型：
--
作者：
Fang, Lan;Shao, Wen;Zeng, Shu-Tang;Tang, Gui-Xue;Yan, Jia-Tong;Chen, Shuo-Bin;Huang, Zhi-Shu;Tan, Jia-Heng;Chen, Xiu-Cai
RNA imaging is of great importance for understanding its complex spatiotemporal dynamics and cellular functions. Considerable effort has been devoted to the development of small-molecule fluorescent probes for RNA imaging. However, most of the reported studies have mainly focused on improving the photostability, permeability, long emission wavelength, and compatibility with live-cell imaging of RNA probes. Less attention has been paid to the selectivity and detection limit of this class of probes. Highly selective and sensitive RNA probes are still rarely available. In this study, a new set of styryl probes were designed and synthesized, with the aim of upgrading the detection limit and maintaining the selectivity of a lead probe QUID−1 for RNA. Among these newly synthesized compounds, QUID−2 was the most promising candidate. The limit of detection (LOD) value of QUID−2 for the RNA was up to 1.8 ng/mL in solution. This property was significantly improved in comparison with that of QUID−1. Further spectroscopy and cell imaging studies demonstrated the advantages of QUID−2 over a commercially available RNA staining probe, SYTO RNASelect, for highly selective and sensitive RNA imaging. In addition, QUID−2 exhibited excellent photostability and low cytotoxicity. Using QUID−2, the global dynamics of RNA were revealed in live cells. More importantly, QUID−2 was found to be potentially applicable for detecting RNA granules in live cells. Collectively, our work provides an ideal probe for RNA imaging. We anticipate that this powerful tool may create new opportunities to investigate the underlying roles of RNA and RNA granules in live cells.
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影响因子：
4.1
作者：
Higuchi, Kei;Sato, Yusuke;Togashi, Nao;Suzuki, Michiyuki;Yoshino, Yukina;Nishizawa, Seiichi
通讯作者：
Nishizawa, Seiichi
影响因子：
14.8
作者：
Bouhedda, Farah;Fam, Kyong Tkhe;Ryckelynck, Michael
通讯作者：
Ryckelynck, Michael
影响因子：
4.9
作者：
Lu, Yu-Jing;Deng, Qiang;Chow, Cheuk-Fai
通讯作者：
Chow, Cheuk-Fai
影响因子：
2.7
作者：
Means, JA;Hines, JV
通讯作者：
Hines, JV
影响因子：
4
作者：
Guo, Lei;Chan, Miu Shan;Wong, Man Shing
通讯作者：
Wong, Man Shing