A Dof transcription factor, SCAP1, is essential for the development of functional stomata in Arabidopsis.
A Dof transcription factor, SCAP1, is essential for the development of functional stomata in Arabidopsis.
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DOI：
10.1016/j.cub.2013.02.001
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发表时间：
2013-03-18
期刊：
影响因子：
9.2
通讯作者：
Iba, Koh
中科院分区：
文献类型：
--
作者：
Negi, Juntaro;Moriwaki, Kosuke;Konishi, Mineko;Yokoyama, Ryusuke;Nakano, Toshiaki;Kusumi, Kensuke;Hashimoto-Sugimoto, Mimi;Schroeder, Julian I.;Nishitani, Kazuhiko;Yanagisawa, Shuichi;Iba, Koh
气孔是高度专业化的器官，由成对的保卫细胞组成，调节植物中的气体和水蒸气交换。虽然保卫细胞分化的早期阶段已经被描述并被解释为类似于动物细胞类型分化过程，但功能性气孔保卫细胞的下游发育仍然知之甚少。我们分离出了拟南芥突变体 scap1（气孔木匠 1），它发育出形状不规则的保卫细胞，并且缺乏控制气孔孔径的能力，包括 CO2 诱导的气孔关闭和光诱导的气孔打开。 SCAP1 被鉴定为植物特有的 Dof 型转录因子，在成熟的保卫细胞中表达，但在保卫母细胞中不表达。 SCAP1 调节编码气孔功能和形态发生关键元件的基因的表达，例如 K+ 通道蛋白、MYB60 转录因子和果胶甲酯酶。因此，scap1保卫细胞中的离子稳态受到干扰，细胞外果胶的酯化作用受到损害，导致孔内的细胞壁无法正常成熟。我们得出结论，SCAP1 调节气孔保卫细胞成熟的基本过程，并作为调节保卫细胞分化最后阶段的关键转录因子发挥作用。
Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
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通讯作者：
Cutler SR
影响因子：
5.1
作者：
Yang, Yingzhen;Costa, Alex;Leonhardt, Nathalie;Siegel, Robert S.;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
7.2
作者：
Plesch, G;Ehrhardt, T;Mueller-Roeber, B
通讯作者：
Mueller-Roeber, B
影响因子：
64.8
作者：
Pillitteri, Lynn Jo;Sloan, Daniel B.;Torii, Keiko U.
通讯作者：
Torii, Keiko U.