PcDWF1, a pear brassinosteroid biosynthetic gene homologous to AtDWARF1, affected the vegetative and reproductive growth of plants
PcDWF1, a pear brassinosteroid biosynthetic gene homologous to AtDWARF1, affected the vegetative and reproductive growth of plants
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PcDWF1是与AtDWARF1同源的梨油菜素类固醇生物合成基因，影响植物的营养和生殖生长
DOI：
10.1186/s12870-020-2323-8
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发表时间：
2020-03
影响因子：
5.3
通讯作者：
Wang Caihong
中科院分区：
文献类型：
--
作者：
Zheng Xiaodong;Xiao Yuxiong;Tian Yike;Yang Shaolan;Wang Caihong
背景
油菜素甾醇（brassinosteroids，BR）是一类重要的甾体激素，在植物生长发育中起着重要的作用.参与BR生物合成的途径和基因主要在模式植物如拟南芥中被鉴定，但对木本水果如梨中的BR生物合成知之甚少。
结果
本研究发现，外源油菜素（BL）能显著提高大梨的茎段生长和生根能力。PcDWF1在矮化型梨中的表达水平显著低于标准型梨，克隆该基因用于进一步分析。系统发育分析表明，PcDWF1是梨油菜素类固醇生物合成基因，与AtDWARF1同源。亚细胞定位分析表明，PcDWF1定位于质膜。在烟草（Nicotiana tabacum）和梨（Pyrus ussuriensis）中过量表达PcDWF1可促进茎的生长，这是由于细胞体积和木质部比对照植株更大，并且生根能力显著增强。除了营养生长的变化外，过表达PcDWF1的烟草植物也比对照烟草植物具有延迟的开花时间和更大的种子大小。这些表型被认为是由于转基因株系中的BL含量高于对照烟草和梨植株。
结论
这些结果表明，梨BR生物合成基因PcDWF1影响了梨和烟草的营养生长和生殖生长，是多年生木本果树中重要的BR生物合成基因。
Background
The steroidal hormones brassinosteroids (BRs) play important roles in plant growth and development. The pathway and genes involved in BR biosynthesis have been identified primarily in model plants like Arabidopsis, but little is known about BR biosynthesis in woody fruits such as pear.
Results
In this study, we found that applying exogenous brassinolide (BL) could significantly increase the stem growth and rooting ability of Pyrus ussuriensis. PcDWF1, which had a significantly lower level of expression in the dwarf-type pear than in the standard-type pear, was cloned for further analysis. A phylogenetic analysis showed that PcDWF1 was a pear brassinosteroid biosynthetic gene that was homologous to AtDWARF1. The subcellular localization analysis indicated that PcDWF1 was located in the plasma membrane. Overexpression of PcDWF1 in tobacco (Nicotiana tabacum) or pear (Pyrus ussuriensis) plants promoted the growth of the stems, which was caused by a larger cell size and more developed xylem than those in the control plants, and the rooting ability was significantly enhanced. In addition to the change in vegetative growth, the tobacco plants overexpressing PcDWF1 also had a delayed flowering time and larger seed size than did the control tobacco plants. These phenotypes were considered to result from the higher BL contents in the transgenic lines than in the control tobacco and pear plants.
Conclusions
Taken together, these results reveal that the pear BR biosynthetic gene PcDWF1 affected the vegetative and reproductive growth of Pyrus ussuriensis and Nicotiana tabacum and could be characterized as an important BR biosynthetic gene in perennial woody fruit plants.
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