Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.).
Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.).
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外源 24-表油菜素内酯与光相互作用调节赤霞珠 (Vitis vinifera L.) 中花青素和原花青素的生物合成
DOI：
10.3390/molecules23010093
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发表时间：
2018-01-09
期刊：
影响因子：
--
通讯作者：
Xi Z
中科院分区：
文献类型：
--
作者：
Zhou Y;Yuan C;Ruan S;Zhang Z;Meng J;Xi Z
花青素和原花青素（PAs）是影响葡萄品质和葡萄酒酿造的关键因素，受到各种刺激和环境因素（糖、激素、光、温度）的刺激。本研究旨在探讨外源24-表油菜素内酯（EBR）和光照对葡萄果实花青素和PAs积累机制的影响。在葡萄株变异开始时，在光照和黑暗条件下（EBR + L, EBR + D）喷洒EBR (0.4 mg/L)，或在光照和黑暗条件下（L， D）喷洒去离子水作为对照。在EBR + L和L处理下，葡萄果皮中积累了大量的花青素，而EBR + D和D处理严重抑制了花青素的积累。这表明EBR处理在光照条件下比在黑暗条件下能产生覆盖效应。实时荧光定量PCR结果显示，光照条件下，EBR上调了VvCHI1、VvCHS2、VvCHS3、VvDFR、VvLDOX、VvMYBA1等基因的表达。在黑暗条件下，只有花青素生物合成的早期基因对EBR有反应。此外，我们还分析了br调控转录因子VvBZR1 （brassinazol -resistant 1）和光调控转录因子VvHY5 （Elongated hypocotyl 5）的表达水平。结果表明，EBR和光照对花青素合成途径相关基因的表达具有协同效应。
Anthocyanins and proanthocyanidins (PAs) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). The aim of the study was to investigate the influence of exogenous 24-Epibrassinolide (EBR) and light on the mechanism of anthocyanins and PAs accumulation in grape berries. Grape clusters were sprayed with EBR (0.4 mg/L) under light and darkness conditions (EBR + L, EBR + D), or sprayed with deionized water under light and darkness conditions as controls (L, D), at the onset of veraison. A large amount of anthocyanins accumulated in the grape skins and was measured under EBR + L and L treatments, whereas EBR + D and D treatments severely suppressed anthocyanin accumulation. This indicated that EBR treatment could produce overlay effects under light, in comparison to that in dark. Real-time quantitative PCR analysis indicated that EBR application up-regulated the expression of genes (VvCHI1, VvCHS2, VvCHS3, VvDFR, VvLDOX, VvMYBA1) under light conditions. Under darkness conditions, only early biosynthetic genes of anthocyanin biosynthesis responded to EBR. Furthermore, we also analyzed the expression levels of the BR-regulated transcription factor VvBZR1 (Brassinazole-resistant 1) and light-regulated transcription factor VvHY5 (Elongated hypocotyl 5). Our results suggested that EBR and light had synergistic effects on the expression of genes in the anthocyanin biosynthesis pathway.
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影响因子：
6.5
作者：
Gagne, Severine;Lacampagne, Soizic;Geny, Laurence
通讯作者：
Geny, Laurence
影响因子：
6.9
作者：
Dai ZW;Meddar M;Renaud C;Merlin I;Hilbert G;Delrot S;Gomès E
通讯作者：
Gomès E
影响因子：
5.7
作者：
Hayat, S.;Ali, B.;Ahmad, A.
通讯作者：
Ahmad, A.
影响因子：
1.2
作者：
Azuma, Akifumi;Fujii, Hiroshi;Yakushiji, Hiroshi
通讯作者：
Yakushiji, Hiroshi
DOI：
10.1111/j.1755-0238.1995.tb00086.x
发表时间：
1995-01-01
影响因子：
2.8
作者：
Coombe, B. G.
通讯作者：
Coombe, B. G.