Evaluation of argon‐induced hydrogen production as a method to measure nitrogen fixation by cyanobacteria
Evaluation of argon‐induced hydrogen production as a method to measure nitrogen fixation by cyanobacteria
复制标题
氩气诱导产氢作为测量蓝藻固氮方法的评估
DOI：
10.1111/jpy.13129
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发表时间：
2021
影响因子：
2.9
通讯作者：
Palenik, ed., B.
中科院分区：
文献类型：
--
作者：
Wilson, Samuel T.;Caffin, Mathieu;White, Angelicque E.;Karl, David M.;Palenik, ed., B.
二氢（H2）的产生是生物二氮（N2）固定的一个神秘而必要的组成部分。本研究调查了固定n2的蓝藻在暴露于空气或由氩气、氧气和二氧化碳（Ar:O2:CO2）组成的气体混合物时对产氢的影响。在没有N2的情况下，氮酶将电子流转向产生H2，这成为衡量总氮酶活性（TNA）的一个指标。与乙炔还原法（AR）相比，这种氩诱导产氢法（AIHP）在推断n2固定速率方面的应用要少得多。我们在这里提供了AIHP方法的全面评估，并展示了其在气体交换流通系统中实现TNA高分辨率测量的能力。尽管缺乏n2，但固定n2的蓝藻仍获得了完整的产氢谱，大致再现了AR试验观察到的时间模式。比较空气和Ar:O2: co2下的H2production揭示了n2固定过程中电子使用的效率，并将这些发现置于细胞代谢的更广泛背景下。最终，AIHP被证明是一种可行的替代AR测定的方法，具有几个额外的优点，可以深入了解细胞生理学，并有望成功应用于现场。
The production of dihydrogen (H2) is an enigmatic yet obligate component of biological dinitrogen (N2) fixation. This study investigates the effect on H2production by N2fixing cyanobacteria when they are exposed to either air or a gas mixture consisting of argon, oxygen, and carbon dioxide (Ar:O2:CO2). In the absence of N2, nitrogenase diverts the flow of electrons to the production of H2, which becomes a measure of Total Nitrogenase Activity (TNA). This method of argon‐induced hydrogen production (AIHP) is much less commonly used to infer rates of N2fixation than the acetylene reduction (AR) assay. We provide here a full evaluation of the AIHP method and demonstrate its ability to achieve high‐resolution measurements of TNA in a gas exchange flow‐through system. Complete diel profiles of H2production were obtained for N2fixing cyanobacteria despite the absence of N2that broadly reproduced the temporal patterns observed by the AR assay. Comparison of H2production under air versus Ar:O2:CO2revealed the efficiency of electron usage during N2fixation and place these findings in the broader context of cell metabolism. Ultimately, AIHP is demonstrated to be a viable alternative to the AR assay with several additional merits that provide an insight into cell physiology and promise for successful field application.
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