Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes.
Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes.
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酵母染色体 I 上的温度敏感致死突变似乎只定义了少数基因。
DOI：
10.1093/genetics/108.1.67
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Pringle,JR
中科院分区：
文献类型：
--
作者：
Kaback,DB;Oeller,PW;YdeSteensma,H;Hirschman,J;Ruezinsky,D;Coleman,KG;Pringle,JR
建立了一种分离酿酒酵母染色体Ⅰ上大量突变的方法。染色体I的菌株单体（即，用甲磺酸乙酯或N-甲基-N ′-硝基-N-亚硝基胍诱变，筛选能在25 ℃但不能在37 ℃的富含葡萄糖的培养基上生长的温度敏感（Ts-）突变体。在染色体I上诱导的隐性突变被表达，而在二倍体染色体上的隐性突变通常不表达，因为同源染色体上存在野生型等位基因。所有染色体上的显性突变也应该表达，但很少出现。- 在分析的41个突变中，32个定位在染色体I上。这32个突变仅属于3个互补组，即先前描述的基因CDC 15、CDC 24和PYK 1（或CDC 19）。我们在CDC 15中发现了16或17个独立突变，在CDC 24中发现了12个独立突变，在PYK 1中发现了3个独立突变。染色体I上的第四个基因MAK 16已知能够产生ats-致死等位基因，但我们在该基因中没有发现突变。使用单体菌株分离的其余9个突变似乎不在染色体I上作图，并且显然在原始突变体中表达，因为它们通过有丝分裂重组或染色体丢失而变成纯合或半合子。- 关于染色体I大小的现有信息表明它应该包含大约60-100个基因。然而，我们在单体菌株中分离出的多个独立等位基因只有三个基因，这表明染色体I上只有一小部分基因容易突变，产生Ts-致死表型。- 在这些研究中，我们将CDC 24定位在染色体I上，并确定它位于染色体左臂PYK 1的远侧着丝粒。
A method was developed for isolating large numbers of mutations on chromosomeIof the yeastSaccharomyces cerevisiae. A strain monosomic for chromosomeI(i.e., haploid for chromosomeIand diploid for all other chromosomes) was mutagenized with either ethyl methanesulfonate orN-methyl-N'-nitro-N-nitrosoguanidine and screened for temperature-sensitive (Ts-) mutants capable of growth on rich, glucose-containing medium at 25° but not at 37°. Recessive mutations induced on chromosomeIare expressed, whereas those on the diploid chromosomes are usually not expressed because of the presence of wild-type alleles on the homologous chromosomes. Dominanttsmutations on all chromosomes should also be expressed, but these appeared rarely. — Of the 41tsmutations analyzed, 32 mapped on chromosomeI. These 32 mutations fell into only three complementation groups, which proved to be the previously described genesCDC15, CDC24andPYK1(orCDC19). We recovered 16 or 17 independent mutations inCDC15, 12 independent mutations inCDC24and three independent mutations inPYK1. A fourth gene on chromosomeI, MAK16, is known to be capable of giving rise to ats-lethal allele, but we recovered no mutations in this gene. The remaining nine mutations isolated using the monosomic strain appeared not to map on chromosomeIand were apparently expressed in the original mutants because they had become homozygous or hemizygous by mitotic recombination or chromosome loss. — The available information about the size of chromosomeIsuggests that it should contain approximately 60-100 genes. However, our isolation in the monosomic strain of multiple, independent alleles of just three genes suggests that only a small proportion of the genes on chromosomeIis easily mutable to give a Ts--lethal phenotype. — During these studies, we locatedCDC24on chromosomeIand determined that it is centromere distal toPYK1on the left arm of the chromosome.
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影响因子：
3.3
作者：
P. Ripoll;A. Garcı́a
通讯作者：
A. Garcı́a
通过整合 DNA 转化对酵母肌动蛋白基因进行致命破坏。
DOI：
10.1126/science.7046050
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shortle,D;Haber,JE;Botstein,D
通讯作者：
Botstein,D
果蝇染色体位点 67B 的发育调控转录。
影响因子：
2.7
作者：
K. Sirotkin;N. Davidson
通讯作者：
N. Davidson
酵母中单体的分离
影响因子：
3.2
作者：
J. Bruenn;R. Mortimer
通讯作者：
R. Mortimer
影响酵母中细胞色素 c 表达的基因：遗传作图和遗传相互作用。
影响因子：
3.3
作者：
R. Rothstein;F. Sherman
通讯作者：
F. Sherman