Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma.
Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma.
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DOI：
10.1038/ijos.2014.32
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发表时间：
2014-12
影响因子：
14.9
通讯作者：
Lin, Li-Min
中科院分区：
文献类型：
--
作者：
Chen, Yuk-Kwan;Huang, Anderson Hsien-Cheng;Lin, Li-Min
先前的研究已经证明，在悬浮培养条件下生长的球状体型细胞在许多癌症中具有癌症干细胞（CSC）特性，但这种现象尚未在VX 2兔口腔癌模型中报道。因此，本研究的目的是研究VX2兔颊鳞状细胞癌（SCC）的球体细胞，并评估其CSC的特性。选用5只成年雄性新西兰白色远缘杂交兔，制备VX 2兔颊黏膜SCC。对VX2兔颊鳞癌标本进行成球细胞培养。自我更新能力;用逆转录-聚合酶链反应（RT-PCR）检测命名簇（CD）44、CD 133、乙醛脱氢酶1（ALDH 1）、B细胞特异性莫洛尼鼠白血病病毒整合位点1（Bmi-1）、巢蛋白、八聚体结合转录因子4（Oct4）和减少表达蛋白-1（雷克斯-1）表达;对顺铂和5-氟尿嘧啶的化学抗性;和裸鼠中球体细胞移植的体内致瘤性以确定所得球体细胞的CSC特征。我们成功地从VX2兔口腔鳞癌组织中获得了球状体细胞。球形细胞具有CSC特征，包括CSC和干细胞标志物的表达（CD 44、Bmi-1、巢蛋白、Oct4和雷克斯-1），在从单解离的球状体细胞重新接种的1周内产生新的球状体集落的能力，化学抗性能力和肿瘤异种移植物的产生（具有类似于原始VX 2兔颊SCC的组织学特征）的103个未分化球体细胞移植到裸鼠中。总之，我们证明了具有CSC细胞特征的球状体细胞可以来源于VX 2兔颊SCC，表明该动物癌症模型适用于研究人类口腔癌中的CSC。
Previous studies have demonstrated that spheroid type cells grown under suspension culture conditions have cancer stem cell (CSC) traits in a number of cancers, but this phenomenon has not yet been reported in the VX2 rabbit oral cancer model. Hence, this study aimed to study the spheroid cells from VX2 rabbit buccal squamous cell carcinomas (SCCs) and assess their CSC characteristics. Five adult male New Zealand white outbred rabbits were used to generate VX2 rabbit buccal SCC. Sphere-forming cell culture was performed for the VX2 rabbit buccal SCC specimens. The self-renewal capability; cluster of designation (CD) 44, CD133, acetaldehyde dehydrogenase 1 (ALDH1), B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1), Nestin, octamer-binding transcription factor 4 (Oct4) and reduced expression protein-1 (Rex-1) expression with reverse transcription-polymerase chain reaction (RT-PCR); chemoresistance to cisplatin and 5-fluorouracil; and in vivo tumorigenicity of spheroid cell transplantation in nude mice were evaluated to determine the CSC characteristics of the resulting spheroid cells. We successfully obtained spheroid cells from the VX2 rabbit OSCC tissues. The spheroid cells exhibited CSC traits, including the expression of CSC and stem cell markers (CD44, Bmi-1, Nestin, Oct4 and Rex-1), capacity to generate new spheroid colonies within 1 week of reseeding from single-dissociated spheroid cells, chemoresistance capacity and generation of tumour xenografts (with histological features resembling those of the original VX2 rabbit buccal SCC) from the transplantation of 103 undifferentiated spheroid cells into nude mice. In summary, we demonstrated that spheroid cells with CSC cell traits can be derived from VX2 rabbit buccal SCCs, indicating that this animal cancer model is applicable for studying CSCs in human oral cancers.
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影响因子：
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