Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models.
Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models.
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DOI：
10.1016/j.jlr.2023.100400
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发表时间：
2023-08
影响因子：
6.5
通讯作者：
Bouwstra, Joke A.
中科院分区：
文献类型：
--
作者：
Nadaban, Andreea;Rousel, Jannik;El Yachioui, Dounia;Gooris, Gerrit S.;Beddoes, Charlotte M.;Dalgliesh, Robert M.;Malfois, Marc;Rissmann, Robert;Bouwstra, Joke A.
皮肤最上层角质层（SC）中的脂质在皮肤屏障功能中起重要作用。SC脂质基质中的三个主要亚类是神经酰胺（CER）、胆固醇和游离脂肪酸。在炎性皮肤病，如特应性皮炎和牛皮癣，SC脂质组成相比，在健康的SC调制。一个主要的变化是CER N-（二十四烷酰基）-鞘氨醇（CER NS）和CER N-（二十四烷酰基）-植物鞘氨醇（CER NP）的浓度之间的摩尔比，这与受损的皮肤屏障功能。在本研究中，我们研究了不同的CER NS：CER NP比例对SC脂质模型系统中脂质组织，脂质排列和屏障功能的影响。结果表明，在患病皮肤中观察到的较高CER NS：CER NP比率并未改变SC中遇到的长周期性阶段中的脂质组织或脂质排列。与CER NS：CER NP 1：2比率（健康皮肤中）相比，CER NS：CER NP 2：1模型（模拟炎性皮肤疾病中的比率）的经表皮水分损失（屏障功能的指示）显著更高。这些发现提供了对健康和患病皮肤中脂质组织的更详细的了解，并表明在体内CER NS：CER NP之间的摩尔比也有助于屏障损伤，但可能不是主要因素。
The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main subclasses in the SC lipid matrix are ceramides (CER), cholesterol, and free fatty acids. In inflammatory skin diseases, such as atopic dermatitis and psoriasis, the SC lipid composition is modulated compared to the composition in healthy SC. One of the main alterations is the molar ratio between the concentration of CER N-(tetracosanoyl)-sphingosine (CER NS) and CER N-(tetracosanoyl)-phytosphingosine (CER NP), which correlated with an impaired skin barrier function. In the present study, we investigated the impact of varying the CER NS:CER NP ratios on the lipid organization, lipid arrangement, and barrier functionality in SC lipid model systems. The results indicate that a higher CER NS:CER NP ratio as observed in diseased skin did not alter the lipid organization or lipid arrangement in the long periodicity phase encountered in SC. The trans-epidermal water loss, an indication of the barrier functionality, was significantly higher for the CER NS:CER NP 2:1 model (mimicking the ratio in inflammatory skin diseases) compared to the CER NS:CER NP 1:2 ratio (in healthy skin). These findings provide a more detailed insight into the lipid organization in both healthy and diseased skin and suggest that in vivo the molar ratio between CER NS:CER NP contributes to barrier impairment as well but might not be the main factor.
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影响因子：
6.5
作者：
BOUWSTRA, JA;GOORIS, GS;BRAS, W
通讯作者：
BRAS, W
影响因子：
6.5
作者：
IMOKAWA, G;ABE, A;HIDANO, A
通讯作者：
HIDANO, A
影响因子：
3.7
作者：
de Jager, Miranda;Groenink, Wouter;Bouwstra, Joke
通讯作者：
Bouwstra, Joke
影响因子：
3.4
作者：
de Jager, Miranda;Groenink, Wouter;Bouwstra, Joke
通讯作者：
Bouwstra, Joke
影响因子：
5.5
作者：
Guo, Shan;Moore, Timothy C.;McCabe, Clare
通讯作者：
McCabe, Clare