Primary blast injury-induced lesions in the retina of adult rats.
Primary blast injury-induced lesions in the retina of adult rats.
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成年大鼠视网膜原发性爆炸损伤引起的病变
DOI：
10.1186/1742-2094-10-79
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发表时间：
2013-07-02
影响因子：
9.3
通讯作者：
Ling EA
中科院分区：
文献类型：
--
作者：
Zou YY;Kan EM;Lu J;Ng KC;Tan MH;Yao L;Ling EA
初级冲击波暴露对大脑的影响已被广泛报道，但其对眼睛的影响尚不清楚。在这里，我们的目标是检查初级冲击波暴露对视网膜的影响。成年雄性SD大鼠暴露于高、低冲击伤后，分别于伤后24、72、2周处死动物。免疫印迹分析血管内皮生长因子(VEGF)、水通道蛋白4(AQP4)、谷氨酰胺合成酶(GS)、诱导型一氧化氮合酶(NOS)、内皮型NOS、神经元型NOS和巢蛋白的表达；细胞因子和趋化因子的ELISA法分析；胶质纤维酸性蛋白(GFAP)/VEGF、GFAP/AQP4、GFAP/Nestin、GS/AQP4、凝集素/iNOS和TUNEL的免疫荧光检测。视网膜中的血管内皮生长因子、诱导型一氧化氮合酶、内皮型一氧化氮合酶、神经元型一氧化氮合酶和巢蛋白的表达随冲击波的严重程度而增加，同时炎性细胞因子和趋化因子的表达也相应增加。初级冲击波暴露后AQP4的表达和视网膜厚度也增加，这与病情严重程度有关。最后观察到TUNEL+和Caspase-3+细胞显著增加。这些变化在伤后24小时即可观察到，并持续到伤后2周。原发母细胞导致视网膜的严重程度相关的病理改变，表现为参与炎症、水肿和细胞凋亡的各种蛋白的表达增加。这些变化是在爆炸暴露后立即观察到的，并持续了两周，表明了急性和慢性损伤机制。这些变化在星形胶质细胞和Müler细胞中最为明显，提示这些细胞在原始细胞后的视网膜病理生理学中起重要作用。
The effect of primary blast exposure on the brain is widely reported but its effects on the eye remains unclear. Here, we aim to examine the effects of primary blast exposure on the retina. Adult male Sprague–Dawley rats were exposed to primary blast high and low injury and sacrificed at 24 h, 72 h, and 2 weeks post injury. The retina was subjected to western analysis for vascular endothelial growth factor (VEGF), aquaporin-4 (AQP4), glutamine synthethase (GS), inducible nitric oxide synthase (NOS), endothelial NOS, neuronal NOS and nestin expression; ELISA analysis for cytokines and chemokines; and immunofluorescence for glial fibrillary acidic protein (GFAP)/VEGF, GFAP/AQP4, GFAP/nestin, GS/AQP4, lectin/iNOS, and TUNEL. The retina showed a blast severity-dependent increase in VEGF, iNOS, eNOS, nNOS, and nestin expression with corresponding increases in inflammatory cytokines and chemokines. There was also increased AQP4 expression and retinal thickness after primary blast exposure that was severity-dependent. Finally, a significant increase in TUNEL+ and Caspase-3+ cells was observed. These changes were observed at 24 h post-injury and sustained up to 2 weeks post injury. Primary blast resulted in severity-dependent pathological changes in the retina, manifested by the increased expression of a variety of proteins involved in inflammation, edema, and apoptosis. These changes were observed immediately after blast exposure and sustained up to 2 weeks suggesting acute and chronic injury mechanisms. These changes were most obvious in the astrocytes and Müller cells and suggest important roles for these cells in retina pathophysiology after blast.
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DOI：
10.1073/pnas.041359198
发表时间：
2001-02-27
影响因子：
11.1
作者：
Fukumura, D;Gohongi, T;Jain, RK
通讯作者：
Jain, RK
DOI：
10.3980/j.issn.2222-3959.2010.03.10
发表时间：
2010-09-18
影响因子：
1.4
作者：
Li, Cai-Rui;Sun, Shu-Guang
通讯作者：
Sun, Shu-Guang
影响因子：
4.2
作者：
Lu, Jia;Ng, Kian Chye;Ling, Eng-Ang
通讯作者：
Ling, Eng-Ang
DOI：
10.1006/bbrc.1998.9719
发表时间：
1998-11-27
影响因子：
3.1
作者：
Kroll, J;Waltenberger, J
通讯作者：
Waltenberger, J
影响因子：
2.5
作者：
BROWN, GC;BOLANOS, JP;CLARK, JB
通讯作者：
CLARK, JB